Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4416890..4417466 Replicon chromosome
Accession NZ_CP086616
Organism Escherichia coli strain RIVM_C039629

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag LN353_RS21315 Protein ID WP_001297643.1
Coordinates 4417179..4417466 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag LN353_RS21310 Protein ID WP_000063160.1
Coordinates 4416890..4417192 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LN353_RS21295 (4413486) 4413486..4415636 + 2151 WP_001340937.1 pyruvate/proton symporter BtsT -
LN353_RS21300 (4415686) 4415686..4415889 + 204 WP_000467859.1 YbdD/YjiX family protein -
LN353_RS21305 (4415900) 4415900..4416856 + 957 WP_001297640.1 GTPase -
LN353_RS21310 (4416890) 4416890..4417192 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
LN353_RS21315 (4417179) 4417179..4417466 - 288 WP_001297643.1 BrnT family toxin Toxin
LN353_RS25780 (4417665) 4417665..4418361 - 697 Protein_4178 IS1 family transposase -
LN353_RS21330 (4418676) 4418676..4419908 + 1233 WP_001137031.1 multidrug efflux MFS transporter MdtM -
LN353_RS21335 (4419949) 4419949..4421229 + 1281 WP_001037390.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4417665..4417874 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T224044 WP_001297643.1 NZ_CP086616:c4417466-4417179 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T224044 NZ_CP119577:1860501-1860755 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT224044 WP_000063160.1 NZ_CP086616:c4417192-4416890 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT224044 NZ_CP119577:1860253-1860504 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References