Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3858905..3859523 Replicon chromosome
Accession NZ_CP086616
Organism Escherichia coli strain RIVM_C039629

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag LN353_RS18690 Protein ID WP_001291435.1
Coordinates 3859305..3859523 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag LN353_RS18685 Protein ID WP_000344800.1
Coordinates 3858905..3859279 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LN353_RS18675 (3853994) 3853994..3855187 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LN353_RS18680 (3855210) 3855210..3858359 + 3150 WP_001132480.1 efflux RND transporter permease AcrB -
LN353_RS18685 (3858905) 3858905..3859279 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
LN353_RS18690 (3859305) 3859305..3859523 + 219 WP_001291435.1 HHA domain-containing protein Toxin
LN353_RS18695 (3859695) 3859695..3860246 + 552 WP_000102564.1 maltose O-acetyltransferase -
LN353_RS18700 (3860362) 3860362..3860832 + 471 WP_000136192.1 YlaC family protein -
LN353_RS18705 (3860996) 3860996..3862546 + 1551 WP_001366446.1 cyclic-guanylate-specific phosphodiesterase PdeB -
LN353_RS18710 (3862588) 3862588..3862941 - 354 WP_085334692.1 DUF1428 family protein -
LN353_RS18720 (3863320) 3863320..3863631 + 312 WP_000409911.1 MGMT family protein -
LN353_RS18725 (3863662) 3863662..3864234 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T224041 WP_001291435.1 NZ_CP086616:3859305-3859523 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T224041 NZ_CP119577:c1142553-1142176 [Escherichia coli]
ATGAACACATTACCCGATACACACGTACGGGAGGCATCGCATTGCCAGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTTACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACTCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT224041 WP_000344800.1 NZ_CP086616:3858905-3859279 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT224041 NZ_CP119577:c1143011-1142643 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAAGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References