Detailed information of TA system TA224040

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3405857..3406562
• Replicon: chromosome
• Accession: NZ_CP086616
• Organism: Escherichia coli strain RIVM_C039629

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: LN353_RS16575
• Protein ID: WP_000539521.1
• Coordinates: 3405857..3406243 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: LN353_RS16580
• Protein ID: WP_001280945.1
• Coordinates: 3406233..3406562 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LN353_RS16555 (3401861)	3401861..3402487	+	627	WP_001295292.1	glutathione S-transferase GstB	-
LN353_RS16560 (3402484)	3402484..3403599	-	1116	WP_000555051.1	aldose sugar dehydrogenase YliI	-
LN353_RS16565 (3403710)	3403710..3404093	-	384	WP_000497137.1	biofilm formation regulator BssR	-
LN353_RS16570 (3404306)	3404306..3405631	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
LN353_RS16575 (3405857)	3405857..3406243	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LN353_RS16580 (3406233)	3406233..3406562	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
LN353_RS16585 (3406632)	3406632..3407960	-	1329	WP_001550887.1	GGDEF domain-containing protein	-
LN353_RS16590 (3407968)	3407968..3410316	-	2349	WP_001550886.1	EAL domain-containing protein	-
LN353_RS16595 (3410494)	3410494..3411405	-	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T224040 WP_000539521.1 NZ_CP086616:3405857-3406243 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T224040 NZ_CP119577:1006084-1006419 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT224040 WP_001280945.1 NZ_CP086616:3406233-3406562 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT224040 NZ_CP119577:1005836-1006084 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6