Detailed information of TA system TA224038

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2773824..2774462
• Replicon: chromosome
• Accession: NZ_CP086616
• Organism: Escherichia coli strain RIVM_C039629

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A7U9DHD1
• Locus tag: LN353_RS13415
• Protein ID: WP_000813795.1
• Coordinates: 2774286..2774462 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LN353_RS13410
• Protein ID: WP_076797675.1
• Coordinates: 2773824..2774240 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LN353_RS13390 (2768976)	2768976..2769917	-	942	WP_001251315.1	ABC transporter permease	-
LN353_RS13395 (2769918)	2769918..2770931	-	1014	WP_001551095.1	ABC transporter ATP-binding protein	-
LN353_RS13400 (2770949)	2770949..2772094	-	1146	WP_001551094.1	ABC transporter substrate-binding protein	-
LN353_RS13405 (2772339)	2772339..2773745	-	1407	WP_001551093.1	PLP-dependent aminotransferase family protein	-
LN353_RS13410 (2773824)	2773824..2774240	-	417	WP_076797675.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
LN353_RS13415 (2774286)	2774286..2774462	-	177	WP_000813795.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
LN353_RS13420 (2774684)	2774684..2774914	+	231	WP_023910283.1	YncJ family protein	-
LN353_RS13425 (2775006)	2775006..2776967	-	1962	WP_001551090.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
LN353_RS13430 (2777040)	2777040..2777576	-	537	WP_001551089.1	DNA-binding transcriptional regulator SutR	-
LN353_RS13435 (2777668)	2777668..2778840	+	1173	WP_001551088.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-65)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6747.80 Da
• Isoelectric Point: 11.5666

>T224038 WP_000813795.1 NZ_CP086616:c2774462-2774286 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPSEEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T224038 NZ_CP119577:c607675-607211 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTTTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15220.55 Da
• Isoelectric Point: 4.7386

>AT224038 WP_076797675.1 NZ_CP086616:c2774240-2773824 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPSNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT224038 NZ_CP119577:c608010-607675 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A7U9DHD1