Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yeeUV (cbtA-cbeA)/CbtA-CbeA |
| Location | 971496..972291 | Replicon | chromosome |
| Accession | NZ_CP086616 | ||
| Organism | Escherichia coli strain RIVM_C039629 | ||
Toxin (Protein)
| Gene name | yeeV | Uniprot ID | A0A4Y9BN12 |
| Locus tag | LN353_RS04745 | Protein ID | WP_023909482.1 |
| Coordinates | 971496..971870 (-) | Length | 125 a.a. |
Antitoxin (Protein)
| Gene name | yeeU | Uniprot ID | A0A4Y9BHM8 |
| Locus tag | LN353_RS04750 | Protein ID | WP_023909483.1 |
| Coordinates | 971917..972291 (-) | Length | 125 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LN353_RS04715 (967461) | 967461..968726 | - | 1266 | WP_001218869.1 | integrase arm-type DNA-binding domain-containing protein | - |
| LN353_RS04720 (969190) | 969190..969516 | - | 327 | WP_001522265.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| LN353_RS04725 (969513) | 969513..969776 | - | 264 | WP_001143297.1 | type II toxin-antitoxin system ParD family antitoxin | - |
| LN353_RS04730 (969848) | 969848..970714 | - | 867 | WP_023909479.1 | DUF4942 domain-containing protein | - |
| LN353_RS04735 (970799) | 970799..970996 | - | 198 | WP_001522268.1 | DUF957 domain-containing protein | - |
| LN353_RS04740 (971008) | 971008..971499 | - | 492 | WP_023909481.1 | DUF5983 family protein | - |
| LN353_RS04745 (971496) | 971496..971870 | - | 375 | WP_023909482.1 | TA system toxin CbtA family protein | Toxin |
| LN353_RS04750 (971917) | 971917..972291 | - | 375 | WP_023909483.1 | type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA | Antitoxin |
| LN353_RS04755 (972371) | 972371..972592 | - | 222 | WP_023909484.1 | DUF987 domain-containing protein | - |
| LN353_RS04760 (972644) | 972644..973399 | - | 756 | WP_001522280.1 | IS21-like element ISEc10 family helper ATPase IstB | - |
| LN353_RS04765 (973396) | 973396..974895 | - | 1500 | WP_059332257.1 | IS21-like element ISEc10 family transposase | - |
| LN353_RS04770 (975076) | 975076..975552 | - | 477 | WP_001186724.1 | RadC family protein | - |
| LN353_RS04775 (975567) | 975567..976040 | - | 474 | WP_001522284.1 | antirestriction protein | - |
| LN353_RS04780 (976135) | 976135..976311 | - | 177 | WP_001522285.1 | hypothetical protein | - |
| LN353_RS04785 (976311) | 976311..977129 | - | 819 | WP_001522287.1 | DUF932 domain-containing protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Integrative and Conjugative Element | - | gspC / gspD / gspE / gspF / gspG / gspH / gspI / gspJ / gspK / gspL / gspM / kpsM / kpsT / ugd / kpsS / kpsC / kpsU / kpsD / kpsE / kpsF / papX | 779743..974819 | 195076 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 125 a.a. Molecular weight: 14009.98 Da Isoelectric Point: 8.2905
>T224025 WP_023909482.1 NZ_CP086616:c971870-971496 [Escherichia coli]
MKTLSDTHVREASRCPSPVTIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRSNYRTVNNITQGKHPEAK
MKTLSDTHVREASRCPSPVTIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRSNYRTVNNITQGKHPEAK
Download Length: 375 bp
>T224025 NZ_CP119571:c1165040-1164888 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACTGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATCTCTGATCAAAATGATCAAGAAAAAAATTCTGAAGAAGACAGTCAGTAA
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACTGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATCTCTGATCAAAATGATCAAGAAAAAAATTCTGAAGAAGACAGTCAGTAA
Antitoxin
Download Length: 125 a.a. Molecular weight: 13598.31 Da Isoelectric Point: 5.5051
>AT224025 WP_023909483.1 NZ_CP086616:c972291-971917 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHPDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAPATTS
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHPDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAPATTS
Download Length: 375 bp
>AT224025 NZ_CP119571:c1165664-1165065 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTTCTAGATGAATTAAAGAAAAATGTTG
AAAAATATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACAGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTTCTAGATGAATTAAAGAAAAATGTTG
AAAAATATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACAGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A4Y9BN12 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A4Y9BHM8 |