Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 896771..897606 Replicon chromosome
Accession NZ_CP086616
Organism Escherichia coli strain RIVM_C039629

Toxin (Protein)


Gene name yeeV Uniprot ID A0A0A1A9A5
Locus tag LN353_RS04335 Protein ID WP_001564063.1
Coordinates 896771..897148 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A0K5N986
Locus tag LN353_RS04340 Protein ID WP_021553056.1
Coordinates 897238..897606 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LN353_RS04305 (891899) 891899..893047 - 1149 WP_000905920.1 capsule polysaccharide export inner-membrane protein KpsE -
LN353_RS04310 (893119) 893119..894102 - 984 WP_001296394.1 KpsF/GutQ family sugar-phosphate isomerase -
LN353_RS04315 (894912) 894912..895082 - 171 Protein_847 IS110 family transposase -
LN353_RS04320 (895425) 895425..896267 - 843 Protein_848 DUF4942 domain-containing protein -
LN353_RS04325 (896352) 896352..896546 - 195 WP_024181941.1 DUF957 domain-containing protein -
LN353_RS04330 (896625) 896625..896774 - 150 Protein_850 DUF5983 family protein -
LN353_RS04335 (896771) 896771..897148 - 378 WP_001564063.1 TA system toxin CbtA family protein Toxin
LN353_RS04340 (897238) 897238..897606 - 369 WP_021553056.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
LN353_RS04345 (897769) 897769..897990 - 222 WP_000692345.1 DUF987 domain-containing protein -
LN353_RS04350 (898055) 898055..898531 - 477 WP_021553055.1 RadC family protein -
LN353_RS04355 (898547) 898547..899026 - 480 WP_001564060.1 antirestriction protein -
LN353_RS04360 (899292) 899292..900110 - 819 WP_001564059.1 DUF932 domain-containing protein -
LN353_RS04365 (900200) 900200..900433 - 234 WP_001278293.1 DUF905 family protein -
LN353_RS04370 (900439) 900439..901116 - 678 WP_001564058.1 hypothetical protein -
LN353_RS04375 (901267) 901267..901947 - 681 WP_001278649.1 WYL domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - gspC / gspD / gspE / gspF / gspG / gspH / gspI / gspJ / gspK / gspL / gspM / kpsM / kpsT / ugd / kpsS / kpsC / kpsU / kpsD / kpsE / kpsF / papX 779743..974819 195076


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14094.09 Da        Isoelectric Point: 7.8045

>T224024 WP_001564063.1 NZ_CP086616:c897148-896771 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRSNYRTVNNITRGKHPEAKQ

Download         Length: 378 bp

>T224024 NZ_CP119571:109702-109953 [Staphylococcus aureus]
ATGAATAACCGCGAACAAATTGAACAGTCCGTTATAAGTGCTAGTGCGTATAACGGTAATGACACAGAGGGATTGCTAAA
AGAGATTGAGGACGTGTATAAGAAAGCACAAGCGTTTGATGAAATACTTGAGGGAATGACAAATGCTATACAACATTCAG
TTAAAGAAGGTGTTGAACTTGATGAAGCAGTAGGGATTATGACGGGGCAAGTGGTCTATAAATATGAGGAGGAGCAGGAA
AATGAGTATTAG

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13615.39 Da        Isoelectric Point: 7.0264

>AT224024 WP_021553056.1 NZ_CP086616:c897606-897238 [Escherichia coli]
VSDTLSGTTHPDDNHDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGNRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Download         Length: 369 bp

>AT224024 NZ_CP119571:109943-110116 [Staphylococcus aureus]
ATGAGTATTAGTGTAGGAGATAAAGTATATAACCATGAAACAAACGAAAGTCTAGAGATTGTGCAATTGGTCGGAGATAT
TAGAGATACACATTATAAACTGTCTGATGATTCAGTTATTAGCATTATAGATTTTATTACTAAACCAATTTATCTAATTA
AGGGGGACGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A1A9A5

Antitoxin

Source ID Structure
AlphaFold DB A0A0K5N986

References