Detailed information of TA system TA224022

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 213552..214164
• Replicon: chromosome
• Accession: NZ_CP086616
• Organism: Escherichia coli strain RIVM_C039629

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: U9YXE2
• Locus tag: LN353_RS00985
• Protein ID: WP_000833473.1
• Coordinates: 213552..213737 (+)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: L4IWR9
• Locus tag: LN353_RS00990
• Protein ID: WP_000499744.1
• Coordinates: 213754..214164 (+)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LN353_RS00975 (210393)	210393..211544	+	1152	WP_000741501.1	L-threonine dehydrogenase	-
LN353_RS00980 (211745)	211745..213283	+	1539	WP_001551836.1	aldehyde dehydrogenase AldB	-
LN353_RS00985 (213552)	213552..213737	+	186	WP_000833473.1	type II toxin-antitoxin system HicA family toxin	Toxin
LN353_RS00990 (213754)	213754..214164	+	411	WP_000499744.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LN353_RS00995 (214236)	214236..216200	-	1965	WP_001551835.1	glycoside hydrolase family 127 protein	-
LN353_RS01000 (216211)	216211..217611	-	1401	WP_000204811.1	MFS transporter	-
LN353_RS01005 (217837)	217837..218652	+	816	WP_000891823.1	AraC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-129)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 6800.89 Da
• Isoelectric Point: 11.7053

>T224022 WP_000833473.1 NZ_CP086616:213552-213737 [Escherichia coli]
VKSADVIAILKQHGWEHIRTRGSHHQFRHPVHPGLVTVPHPKKDIKPGTLAQIGRQAGLTF

Nucleotide

• Length: 186 bp

>T224022 NZ_CP119568:477357-477758 [Pararhizobium sp. T808]
ATGCTGAAGTACATGTTAGACACCAACATTTGCATTTTCACGATCAAAAATCGACCGCAGCAGGTGCGCGAGGCGTTCAA
TCGTTTCCATGATCAATTGTGCATCAGTTCGGTTAGCTTGATGGAACTGATTTACGGAGCGGAAAAGTCAGCCAGTCCTG
AAAAGAACCTTCCGGTTGTGGAGGGCTTCGCGGCGCGCCTTGAAGTTCTAGCGTACGACGAACCGGCAGCAAGCCATACG
GGACAACTGCGGGCCGAACTAGCGCGCAGCGGTACTCCAATCGGTCCATATGATCAATTGATTGCAGGACACGCTCGCTC
GCGAGGCTTGATTGTGGTTACGAATAATCGCAGGGAATTTGATCGCGTTCCCGGGTTACGGGTGGAAGACTGGACGGGCT
AA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 15241.15 Da
• Isoelectric Point: 4.5486

>AT224022 WP_000499744.1 NZ_CP086616:213754-214164 [Escherichia coli]
MFYPAYIHSDPDGSASGFFPDVPGCYFAGDTLDNAFQDARSALVAHFETLCEMNEELPLPGSVETHLVQRAQDFIGGQWL
LVDINMNQFEGRAERINITMPKRLLNKIDTYVRNNPDYANRSAFLAEAARRVLPGV

Nucleotide

• Length: 411 bp

>AT224022 NZ_CP119568:477127-477357 [Pararhizobium sp. T808]
GTGGAGCAGGGTGCAGTATTCCAGAATAATCGAAGCCAAGCTATCCGTCTTCCAAAGGCAGTCGCTTTGCCCGATGATGT
AAAGCGCGTCGATATTGTCGCCGTCGGACGCACTCGCATTATCGCGCCTGCTGGCGAGGTCTGGGACAGCTGGTTTGAAG
GAGCAGCGGCCACGTCCGACTTCATGAGTGAGCGTGATCAGGCCGCCTCGCAGGAGCGCGAGGCGTTTTGA

Structures

Toxin

• AlphaFold DB: U9YXE2

Antitoxin

• AlphaFold DB: L4IWR9