Detailed information of TA system TA224020

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3373071..3373714
• Replicon: chromosome
• Accession: NZ_CP086615
• Organism: Spiribacter sp. E85

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LMH63_RS15775
• Protein ID: WP_109678914.1
• Coordinates: 3373313..3373714 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LMH63_RS15770
• Protein ID: WP_109678913.1
• Coordinates: 3373071..3373307 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMH63_RS15735 (LMH63_15735)	3368826..3369176	-	351	WP_109678908.1	hypothetical protein	-
LMH63_RS15740	3369225..3369554	-	330	WP_109678909.1	DUF2589 domain-containing protein	-
LMH63_RS15745	3369551..3369973	-	423	WP_109678910.1	DUF2589 domain-containing protein	-
LMH63_RS15750 (LMH63_15745)	3369996..3370778	-	783	WP_229332802.1	DUF4332 domain-containing protein	-
LMH63_RS15755 (LMH63_15750)	3370868..3371014	+	147	WP_199225672.1	hypothetical protein	-
LMH63_RS15760 (LMH63_15755)	3371226..3372449	-	1224	WP_109678911.1	type II toxin-antitoxin system HipA family toxin	-
LMH63_RS15765 (LMH63_15760)	3372442..3372759	-	318	WP_109678912.1	helix-turn-helix transcriptional regulator	-
LMH63_RS15770 (LMH63_15765)	3373071..3373307	+	237	WP_109678913.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LMH63_RS15775 (LMH63_15770)	3373313..3373714	+	402	WP_109678914.1	type II toxin-antitoxin system VapC family toxin	Toxin
LMH63_RS15780 (LMH63_15775)	3373955..3374353	-	399	WP_109678915.1	type II toxin-antitoxin system VapC family toxin	-
LMH63_RS15785 (LMH63_15780)	3374350..3374580	-	231	WP_109678916.1	prevent-host-death protein	-
LMH63_RS15790 (LMH63_15785)	3375162..3376466	+	1305	WP_109678917.1	hypothetical protein	-
LMH63_RS15795 (LMH63_15790)	3376574..3376771	-	198	WP_109678918.1	DUF2283 domain-containing protein	-
LMH63_RS15800 (LMH63_15795)	3376768..3376973	-	206	Protein_3118	hypothetical protein	-
LMH63_RS15805 (LMH63_15800)	3377108..3377845	-	738	WP_109678919.1	type III pantothenate kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-123)

Antitoxin

• SpoVT-AbrB domain (8-44)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14687.92 Da
• Isoelectric Point: 7.2781

>T224020 WP_109678914.1 NZ_CP086615:3373313-3373714 [Spiribacter sp. E85]
VAFLLDTNTCIYIINRRPPEVFERFRGHAIGEIGISSITGAELTFGVAKSGSRRNRAALDKFLAPLDILPFDEAAMRQYG
PLRAHLEREGQPIGSMDLLIAAHALALGYTLITNNLREFARVPNLPLENWVGS

Nucleotide

• Length: 402 bp

>T224020 NZ_CP119567:c625659-625228 [Pararhizobium sp. T808]
ATGATCTTGCTTGATACCAACGTGATATCGGAGCCTTGGAAACCGGCCCCTGACGAGACAGTGGTCGCCTGGCTGGACGC
GCAAGCAGTTGAGACGCTGTTTATCTCAGCGATAACAATTGCCGAACTGCGTTTTGGGATCGCCGCCATGCCCGCTGGGA
AACGGCAAACTATTCTCCGTGATCGCCTGGAGGTCGAAGTGCTTCCGCATTTTGCGGGGCGTATCCTGCCTTTTGGCGTT
GCCACGTCGCAGTTTTATTCGGAACTGATGGTGCGCGCTCGGTTGTCCGGTAAGGCGATCGGCGAGGCCGATGGATATAT
TGCAGCCACGGCAGCAGAAAACCGTCTTGCGGTCGCAACCCGCGACATCAATCCTTTCGAGGCTGCTGGGTTGAAGGTGA
TCAACCCTTGGATCCTACAGCAATTAAGTTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8961.20 Da
• Isoelectric Point: 4.5358

>AT224020 WP_109678913.1 NZ_CP086615:3373071-3373307 [Spiribacter sp. E85]
MATTAKIFTSGRSQAVRLPKSLRFEGTEVIAKRFGNGVLLLPVDAPWEVMQEALEEFEPDFQMGREQPPLQDRDNWPW

Nucleotide

• Length: 237 bp

>AT224020 NZ_CP119567:c625910-625656 [Pararhizobium sp. T808]
ATGCCGGCTGTTATCATTCGCAATCTGTCCGAGGAAACGCATCGCGCTTTAAAGGTGCGAGCAGCTCACCATGGCCGAAG
CACCGAGGCGGAAATCAGGGACATTATTGAAGCGGCTGTTCGTCCCGCTGAGCGTGTCAAACTCGGGTCTTTACTGGCGG
CCATCGGCCGTGACGCGGAGCTTTCTGACACCGATGTTGAAGTGCTACAGCAAAACCGCGGTAAGGCACCCGCCGAGCCT
ATGACATTCGAATGA