Detailed information of TA system TA224017

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii(toxin)
• Location: 307852..309199
• Replicon: plasmid unnamed2
• Accession: NZ_CP086612
• Organism: Shinella zoogloeoides strain ATCC 19623

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: -
• Locus tag: K8M09_RS22665
• Protein ID: WP_229342524.1
• Coordinates: 308345..309199 (+)
• Length: 285 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: K8M09_RS22660
• Protein ID: WP_229342523.1
• Coordinates: 307852..308352 (+)
• Length: 167 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8M09_RS22635 (K8M09_22630)	304137..304340	+	204	Protein_280	SAVED domain-containing protein	-
K8M09_RS22640 (K8M09_22635)	304442..304966	+	525	WP_229342519.1	DUF2235 domain-containing protein	-
K8M09_RS22645 (K8M09_22640)	304979..306210	+	1232	WP_160788269.1	IS3 family transposase	-
K8M09_RS22650 (K8M09_22645)	306289..306633	+	345	WP_229342521.1	hypothetical protein	-
K8M09_RS22660 (K8M09_22655)	307852..308352	+	501	WP_229342523.1	transcriptional regulator	Antitoxin
K8M09_RS22665 (K8M09_22660)	308345..309199	+	855	WP_229342524.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
K8M09_RS22670 (K8M09_22665)	309300..310754	+	1455	WP_229341921.1	IS21 family transposase	-
K8M09_RS22675 (K8M09_22670)	310751..311509	+	759	WP_229341825.1	IS21-like element helper ATPase IstB	-
K8M09_RS22680 (K8M09_22675)	311644..312248	-	605	Protein_289	helix-turn-helix domain-containing protein	-
K8M09_RS22685 (K8M09_22680)	312461..313021	-	561	Protein_290	IS21-like element helper ATPase IstB	-
K8M09_RS22690 (K8M09_22685)	313020..313130	+	111	Protein_291	Hsp20 family protein	-
K8M09_RS22695 (K8M09_22690)	313563..313937	-	375	WP_160787931.1	DUF779 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	katB	1..340407	340407
	-	inside	IScluster/Tn	-	-	297497..312248	14751

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (38-259)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 285 a.a.
• Molecular weight: 31889.37 Da
• Isoelectric Point: 5.2816

>T224017 WP_229342524.1 NZ_CP086612:308345-309199 [Shinella zoogloeoides]
MAKEVRNVGASVRARLLQLAKTSGQSFDLVLTRFALERLLFRLSRSPHAGRFVLKGAMLMMSWFEDPHRGTRDLDLLGFG
NPEPDPMLETFREILAQEVDDGVMFDADTLRVDRIREELEYGGLRLRAVASISGARINLTIDIGFGDALEPGAEMLDYPS
MLNFPAPRLRAYARETVIAEKFQAMVMLGRVNSRMKDFYDIWILSRSFDFDDDRLARAIAATFERRETPIPEDLPDALTD
AFAKDEQKQRQWRAFVEGVAHNPGESGGAKVGHGSGGIIQPRAE

Nucleotide

• Length: 855 bp

>T224017 NZ_CP119566:c3589601-3589311 [Pararhizobium sp. T808]
ATGCTCGTATTTGAAACAACCGATGTGTTCAAGGATTGGCTGTTTTCGCTGCGGGACGTGAAGGCGAAGGCCAGAATTCT
TGCGCGGATACGCTCCGCCGAGTTCGGCAATCTGGGAGACTGCGCACCGGTTGGCGAGGGTGTCTCGGAAATGCGGATCC
ATTATGGACCAGGGTATCGCTTGTATTTCAAACGGCGTGGCGACGTTGTCTATCTCCTACTCGCCGGGGGCGACAAGTCC
AGCCAGAAGCGGGACATAGCAGCCGCATTGAAGATGGCGAAGTCGCTTTAG

Antitoxin

Protein

• Length: 167 a.a.
• Molecular weight: 18158.85 Da
• Isoelectric Point: 8.5307

>AT224017 WP_229342523.1 NZ_CP086612:307852-308352 [Shinella zoogloeoides]
MGRTLIHSGGNSQWQVTDAELDANHSLAEIAKRVPKAVVCLVSALAFHGLTDQLPRHVWLAIGRKDWAPKPDGTPIRIVR
FTESLLNESVETHVIEGVPVKVFEIAKTIADCFRYRSKIGLSVAIEGLQEALRQRKTTPSEIANQAERGGVGTVIRPYIE
ALTANG

Nucleotide

• Length: 501 bp

>AT224017 NZ_CP119566:c3589299-3589006 [Pararhizobium sp. T808]
ATGACAACGGTCTCCAGATTTGACGCATCCGAGTTTCTCGATGACGATGACCTTGTCGCCGAATATCTCACGGCTGCGAT
GGAAGATGACAATCCCGATGTCTTCATCGCAGCTCTGGGTGATGTCGCCAAGGCGCGCGGAATGACTGAGATTGCGAGGG
CCTCGGGGCTTGGCCGTGAAAGCCTCTACAAGTCGCTGCGGGGCGGATCGAAGGCCCGGTTTGAAACGCTCCACAAGATC
GTTAATACGCTCGGGTTGAAGATGACGATTGTTGCCGATCGCCCAACACCTTGA