Detailed information of TA system TA224016

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 121355..121992
• Replicon: plasmid unnamed1
• Accession: NZ_CP086611
• Organism: Shinella zoogloeoides strain ATCC 19623

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: K8M09_RS20165
• Protein ID: WP_160785771.1
• Coordinates: 121591..121992 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: VapB1
• Uniprot ID: -
• Locus tag: K8M09_RS20160
• Protein ID: WP_160785772.1
• Coordinates: 121355..121591 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8M09_RS20130 (K8M09_20125)	116695..117177	-	483	Protein_97	ABC transporter ATP-binding protein	-
K8M09_RS20135 (K8M09_20130)	117174..117962	-	789	WP_160785777.1	ABC transporter permease	-
K8M09_RS20140 (K8M09_20135)	117966..118829	-	864	WP_160785776.1	ABC transporter permease	-
K8M09_RS20145 (K8M09_20140)	119054..120130	-	1077	WP_160785775.1	spermidine/putrescine ABC transporter substrate-binding protein	-
K8M09_RS20150 (K8M09_20145)	120259..120885	+	627	WP_160785774.1	TetR/AcrR family transcriptional regulator	-
K8M09_RS20155 (K8M09_20150)	120900..121292	+	393	WP_160785773.1	nuclear transport factor 2 family protein	-
K8M09_RS20160 (K8M09_20155)	121355..121591	+	237	WP_160785772.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
K8M09_RS20165 (K8M09_20160)	121591..121992	+	402	WP_160785771.1	type II toxin-antitoxin system VapC family toxin	Toxin
K8M09_RS20170 (K8M09_20165)	121993..122403	-	411	WP_160785770.1	Cu(I)-responsive transcriptional regulator	-
K8M09_RS20175 (K8M09_20170)	122400..124862	-	2463	WP_160785769.1	heavy metal translocating P-type ATPase	-
K8M09_RS20180 (K8M09_20175)	124937..125149	-	213	WP_160785768.1	heavy-metal-associated domain-containing protein	-
K8M09_RS20185 (K8M09_20180)	125210..125677	-	468	WP_160785767.1	MarR family winged helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..372552	372552

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-115)

Antitoxin

• SpoVT-AbrB domain (7-43)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14823.02 Da
• Isoelectric Point: 5.1751

>T224016 WP_160785771.1 NZ_CP086611:121591-121992 [Shinella zoogloeoides]
MPRYMLDTNMCIYLMKNQPEAVARRFAACQVGDVVMSAITYAELQYGVAVARDPAREGGNLKDLVELIPVLPFDRLAGDA
YGPARLATRERRSDHLDKLIAAHAIAADAVLVTNNIRDFTAYPDLRLENWLDS

Nucleotide

• Length: 402 bp

>T224016 NZ_CP119566:c3589299-3589006 [Pararhizobium sp. T808]
ATGACAACGGTCTCCAGATTTGACGCATCCGAGTTTCTCGATGACGATGACCTTGTCGCCGAATATCTCACGGCTGCGAT
GGAAGATGACAATCCCGATGTCTTCATCGCAGCTCTGGGTGATGTCGCCAAGGCGCGCGGAATGACTGAGATTGCGAGGG
CCTCGGGGCTTGGCCGTGAAAGCCTCTACAAGTCGCTGCGGGGCGGATCGAAGGCCCGGTTTGAAACGCTCCACAAGATC
GTTAATACGCTCGGGTTGAAGATGACGATTGTTGCCGATCGCCCAACACCTTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8922.95 Da
• Isoelectric Point: 5.8507

>AT224016 WP_160785772.1 NZ_CP086611:121355-121591 [Shinella zoogloeoides]
MHKTRVFRNGNSQAVRIPSDLAYDNTDVEMEIERVGDELRIRPARRSLAGVLDRFARFSPDFMAEGRGEQGQAERDTL

Nucleotide

• Length: 237 bp

>AT224016 NZ_CP119566:c3589601-3589311 [Pararhizobium sp. T808]
ATGCTCGTATTTGAAACAACCGATGTGTTCAAGGATTGGCTGTTTTCGCTGCGGGACGTGAAGGCGAAGGCCAGAATTCT
TGCGCGGATACGCTCCGCCGAGTTCGGCAATCTGGGAGACTGCGCACCGGTTGGCGAGGGTGTCTCGGAAATGCGGATCC
ATTATGGACCAGGGTATCGCTTGTATTTCAAACGGCGTGGCGACGTTGTCTATCTCCTACTCGCCGGGGGCGACAAGTCC
AGCCAGAAGCGGGACATAGCAGCCGCATTGAAGATGGCGAAGTCGCTTTAG