Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 20289..20814 Replicon plasmid pEF45-5
Accession NZ_CP086609
Organism Escherichia fergusonii strain EF20JDJ4045

Toxin (Protein)


Gene name ccdB Uniprot ID F0JY91
Locus tag LNN29_RS25060 Protein ID WP_001159869.1
Coordinates 20289..20594 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag LNN29_RS25065 Protein ID WP_000813630.1
Coordinates 20596..20814 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN29_RS25030 (LNN29_24980) 15770..16204 + 435 WP_000525034.1 IS200/IS605 family transposase -
LNN29_RS25035 (LNN29_24985) 16416..17789 + 1374 WP_000244368.1 IS4-like element ISEc13 family transposase -
LNN29_RS25040 (LNN29_24990) 17889..18938 - 1050 Protein_17 IS4 family transposase -
LNN29_RS25045 (LNN29_24995) 18938..19075 - 138 Protein_18 Lrp/AsnC family transcriptional regulator -
LNN29_RS25050 (LNN29_25000) 19192..20103 + 912 WP_000549737.1 DMT family transporter -
LNN29_RS25055 (LNN29_25005) 20088..20288 - 201 Protein_20 phage integrase family protein -
LNN29_RS25060 (LNN29_25010) 20289..20594 - 306 WP_001159869.1 type II toxin-antitoxin system toxin CcdB Toxin
LNN29_RS25065 (LNN29_25015) 20596..20814 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
LNN29_RS25070 (LNN29_25020) 21873..22301 + 429 Protein_23 hypothetical protein -
LNN29_RS25075 (LNN29_25025) 22391..22987 + 597 Protein_24 IS66 family transposase -
LNN29_RS25080 (LNN29_25030) 23265..23915 - 651 Protein_25 transposase -
LNN29_RS25085 (LNN29_25035) 24793..25023 + 231 WP_252990174.1 IS66 family insertion sequence element accessory protein TnpB -
LNN29_RS25275 24976..25126 + 151 Protein_27 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..46074 46074
- inside IScluster/Tn - - 15770..25974 10204


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11678.46 Da        Isoelectric Point: 6.4674

>T224012 WP_001159869.1 NZ_CP086609:c20594-20289 [Escherichia fergusonii]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTSDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T224012 NZ_CP119566:c3166007-3165729 [Pararhizobium sp. T808]
ATGATCAAATCGATCGCCAATACCGCGACACGGCAATTTACAGAGAGCGGAAAATCAAAGTTTTCAGGCTTGGATGCCGG
CAAGGCAAAAATGCGGCTGGTCATGTTGCAGAATGCCCGGTCGCTTGACGATCTGGCTCCTCTGAAATCGGTTGGTTTGC
ATAAGTTGTCGGGCGATCGAAAAAACCAATGGGCCATGACCATCAATGGGCCTTGGCGGCTGTGTTTCCGATTTGAAGAT
GGGCACGCTTATGACGTGGAAATCGTCGATTACCACTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT224012 WP_000813630.1 NZ_CP086609:c20814-20596 [Escherichia fergusonii]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT224012 NZ_CP119566:c3165717-3165430 [Pararhizobium sp. T808]
ATGTTTGAGGTTCACCCAGGCGCGATGCTAAGAGAAGAATTGGATGCGCGCAGCATGACAGGCAACGCGTTGGCCCTCGC
GTTGCGCATAAACTCCGGCCGGATTTCCGAAATTCTGACTGGAAAACGGGCTATTTCCGCCGATACCGCTTTGAGGCTCG
GTCAGTTCTTTGGAAATGATCCTCAGTTCTGGCTCGGACTTCAGGCCACGTATGATCTCGCGGTCGCGTCGCGGCAAAAC
GGGGATCGGATCAAGGCCGAAGTTCTTCCTGCAAAATCTGCCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3V2SIB9

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References