Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | hicAB/- |
| Location | 8899..9491 | Replicon | plasmid pEF45-4 |
| Accession | NZ_CP086608 | ||
| Organism | Escherichia fergusonii strain EF20JDJ4045 | ||
Toxin (Protein)
| Gene name | hicA | Uniprot ID | A0A1I9ZJC5 |
| Locus tag | LNN29_RS24655 | Protein ID | WP_000513532.1 |
| Coordinates | 8899..9147 (+) | Length | 83 a.a. |
Antitoxin (Protein)
| Gene name | hicB | Uniprot ID | A0A1I9ZJ49 |
| Locus tag | LNN29_RS24660 | Protein ID | WP_000681609.1 |
| Coordinates | 9144..9491 (+) | Length | 116 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LNN29_RS24610 (LNN29_24560) | 3911..4576 | - | 666 | WP_000532149.1 | division plane positioning ATPase MipZ | - |
| LNN29_RS24615 (LNN29_24565) | 4800..5033 | + | 234 | WP_057520283.1 | hypothetical protein | - |
| LNN29_RS24620 (LNN29_24570) | 5165..5494 | + | 330 | WP_001542022.1 | hypothetical protein | - |
| LNN29_RS24625 (LNN29_24575) | 5895..6410 | + | 516 | WP_001025393.1 | J domain-containing protein | - |
| LNN29_RS24630 (LNN29_24580) | 6514..7056 | + | 543 | WP_001083904.1 | hypothetical protein | - |
| LNN29_RS24635 (LNN29_24585) | 7053..7307 | + | 255 | WP_001230709.1 | hypothetical protein | - |
| LNN29_RS24640 (LNN29_24590) | 7304..7546 | + | 243 | WP_000151456.1 | hypothetical protein | - |
| LNN29_RS24645 (LNN29_24595) | 7767..7985 | + | 219 | WP_154101533.1 | hypothetical protein | - |
| LNN29_RS24650 (LNN29_24600) | 8117..8410 | + | 294 | WP_000673428.1 | hypothetical protein | - |
| LNN29_RS24655 (LNN29_24605) | 8899..9147 | + | 249 | WP_000513532.1 | type II toxin-antitoxin system HicA family toxin | Toxin |
| LNN29_RS24660 (LNN29_24610) | 9144..9491 | + | 348 | WP_000681609.1 | type II toxin-antitoxin system HicB family antitoxin | Antitoxin |
| LNN29_RS24665 (LNN29_24615) | 9579..10022 | - | 444 | WP_023223351.1 | hypothetical protein | - |
| LNN29_RS24670 (LNN29_24620) | 10263..10562 | + | 300 | WP_034169410.1 | type II toxin-antitoxin system HicB family antitoxin | - |
| LNN29_RS24675 (LNN29_24625) | 10822..11148 | - | 327 | WP_000206697.1 | hypothetical protein | - |
| LNN29_RS24680 (LNN29_24630) | 11150..11473 | - | 324 | WP_223666488.1 | hypothetical protein | - |
| LNN29_RS24685 (LNN29_24635) | 11450..11590 | - | 141 | Protein_21 | hypothetical protein | - |
| LNN29_RS24690 (LNN29_24640) | 11610..12317 | - | 708 | WP_000108794.1 | hypothetical protein | - |
| LNN29_RS24695 (LNN29_24645) | 12319..13071 | - | 753 | WP_000797966.1 | conjugal transfer protein TraL | - |
| LNN29_RS24700 (LNN29_24650) | 13089..13490 | - | 402 | WP_021575837.1 | hypothetical protein | - |
| LNN29_RS24705 (LNN29_24655) | 13858..14199 | + | 342 | WP_000730722.1 | plasmid mobilization relaxosome protein MobC | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Mobilizable plasmid | mcr-1.1 | - | 1..61140 | 61140 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 83 a.a. Molecular weight: 9378.03 Da Isoelectric Point: 10.4800
>T224011 WP_000513532.1 NZ_CP086608:8899-9147 [Escherichia fergusonii]
MGKADKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
VL
MGKADKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
VL
Download Length: 249 bp
>T224011 NZ_CP119566:c2579320-2578892 [Pararhizobium sp. T808]
ATGACGTTTCTGCTGGACGTCAACGTGCTCATCGCTTTGGCAGACAGTAGCCACAGTCATTATTCCGTTGCCACAAGCTG
GTTTGAAAAAATCGGCCAAAAGGATTGGGCCACCTGCCCGATAACCGAAAATGGTATGATCCGTATCCTTTCGGATCCTC
GTTATGGCAGCCCCGTGCCAGATGCCGCAATGGCGACCGAACTTCTCCAGCTATTCCGGCTTACCGGCAAACATTCGTTC
TGGCATGACGACATCAGTCTCACCGACCCCGGCATTTTTATCCGGGATAAAGTCACCTCGAAACATACCACAGATATCTA
TCTGCTTGGACTTGCGAAAGCGCACGGCGGACGGCTTGCCACCTTCGACCGCCGCCTCGCCACACAGGCTGTCATCGGTG
GCTCAGACATCCTTTGCCTGCTCGCCTAG
ATGACGTTTCTGCTGGACGTCAACGTGCTCATCGCTTTGGCAGACAGTAGCCACAGTCATTATTCCGTTGCCACAAGCTG
GTTTGAAAAAATCGGCCAAAAGGATTGGGCCACCTGCCCGATAACCGAAAATGGTATGATCCGTATCCTTTCGGATCCTC
GTTATGGCAGCCCCGTGCCAGATGCCGCAATGGCGACCGAACTTCTCCAGCTATTCCGGCTTACCGGCAAACATTCGTTC
TGGCATGACGACATCAGTCTCACCGACCCCGGCATTTTTATCCGGGATAAAGTCACCTCGAAACATACCACAGATATCTA
TCTGCTTGGACTTGCGAAAGCGCACGGCGGACGGCTTGCCACCTTCGACCGCCGCCTCGCCACACAGGCTGTCATCGGTG
GCTCAGACATCCTTTGCCTGCTCGCCTAG
Antitoxin
Download Length: 116 a.a. Molecular weight: 13206.97 Da Isoelectric Point: 4.8313
>AT224011 WP_000681609.1 NZ_CP086608:9144-9491 [Escherichia fergusonii]
MKHLKYKGYLGTVEPDFENNILYGKLAFIRDLVTYEAETLADLEREFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSHHRAGA
MKHLKYKGYLGTVEPDFENNILYGKLAFIRDLVTYEAETLADLEREFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSHHRAGA
Download Length: 348 bp
>AT224011 NZ_CP119566:c2579571-2579317 [Pararhizobium sp. T808]
ATGCAAACAACGTTGAAGATCGATGATGACGTGCTGCTTGCTGCGCAGCAGCTTGCCGATCGCAATGGTCTTGCAATCGA
TCAGATCATTTCGGATATGGCGCGCCGCGGCATGGGGATGTCTGCTTACATCTCGCAACGCAACGGTATTCCCGTCTTGA
TTCGTCCTGGGGCTCAAATCGTCACGGTGGAAATGGTCGATGCTCTGCGTGATGAAGATTATGACTATTTCTCAAGCGAC
GCTGGCGACAAATGA
ATGCAAACAACGTTGAAGATCGATGATGACGTGCTGCTTGCTGCGCAGCAGCTTGCCGATCGCAATGGTCTTGCAATCGA
TCAGATCATTTCGGATATGGCGCGCCGCGGCATGGGGATGTCTGCTTACATCTCGCAACGCAACGGTATTCCCGTCTTGA
TTCGTCCTGGGGCTCAAATCGTCACGGTGGAAATGGTCGATGCTCTGCGTGATGAAGATTATGACTATTTCTCAAGCGAC
GCTGGCGACAAATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A1I9ZJC5 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A1I9ZJ49 |