22401

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 57506..58031 Replicon plasmid pETEC_74
Accession NC_009790
Organism Escherichia coli E24377A

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag ECE24377A_RS01520 Protein ID WP_001159871.1
Coordinates 57506..57811 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag ECE24377A_RS01525 Protein ID WP_000813634.1
Coordinates 57813..58031 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECE24377A_RS01495 53749..54453 - 705 WP_001119661.1 helix-turn-helix domain-containing protein -
ECE24377A_RS01500 54701..55381 + 681 WP_000452091.1 EAL domain-containing protein -
ECE24377A_RS01505 55821..56462 - 642 WP_000470631.1 recombinase family protein -
ECE24377A_RS01515 56792..57505 - 714 WP_000016957.1 site-specific integrase -
ECE24377A_RS01520 57506..57811 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
ECE24377A_RS01525 57813..58031 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
ECE24377A_RS01530 58476..58733 + 258 WP_000343092.1 hypothetical protein -
ECE24377A_RS01535 58733..59263 + 531 WP_011387704.1 hypothetical protein -
ECE24377A_RS01540 59507..60349 + 843 WP_160371899.1 polysaccharide deacetylase family protein -
ECE24377A_RS26635 60352..61440 + 1089 WP_004100285.1 putative hexosyltransferase CapU -
ECE24377A_RS01550 61445..62395 + 951 WP_012000752.1 virulence factor VirK -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..74224 74224
- flank IS/Tn - - 55821..56444 623


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T22401 WP_001159871.1 NC_009790:c57811-57506 [Escherichia coli O139:H28 str. E24377A]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T22401 NC_009790:c57811-57506 [Escherichia coli O139:H28 str. E24377A]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTACTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT22401 WP_000813634.1 NC_009790:c58031-57813 [Escherichia coli O139:H28 str. E24377A]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT22401 NC_009790:c58031-57813 [Escherichia coli O139:H28 str. E24377A]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACCACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CQY2

References