22401

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 57506..58031 Replicon plasmid pETEC_74
Accession NC_009790
Organism Escherichia coli E24377A

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag ECE24377A_RS01520 Protein ID WP_001159871.1
Coordinates 57506..57811 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag ECE24377A_RS01525 Protein ID WP_000813634.1
Coordinates 57813..58031 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECE24377A_RS01495 53749..54453 - 705 WP_001119661.1 helix-turn-helix domain-containing protein -
ECE24377A_RS01500 54701..55381 + 681 WP_000452091.1 EAL domain-containing protein -
ECE24377A_RS01505 55821..56462 - 642 WP_000470631.1 recombinase family protein -
ECE24377A_RS01515 56792..57505 - 714 WP_000016957.1 site-specific integrase -
ECE24377A_RS01520 57506..57811 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
ECE24377A_RS01525 57813..58031 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
ECE24377A_RS01530 58476..58733 + 258 WP_000343092.1 hypothetical protein -
ECE24377A_RS01535 58733..59263 + 531 WP_011387704.1 hypothetical protein -
ECE24377A_RS01540 59507..60349 + 843 WP_160371899.1 polysaccharide deacetylase family protein -
ECE24377A_RS26635 60352..61440 + 1089 WP_004100285.1 putative hexosyltransferase CapU -
ECE24377A_RS01550 61445..62395 + 951 WP_012000752.1 virulence factor VirK -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..74224 74224
- flank IS/Tn - - 55821..56444 623


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T22401 WP_001159871.1 NC_009790:c57811-57506 [Escherichia coli O139:H28 str. E24377A]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T22401 NC_009790:c57811-57506 [Escherichia coli O139:H28 str. E24377A]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTACTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT22401 WP_000813634.1 NC_009790:c58031-57813 [Escherichia coli O139:H28 str. E24377A]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT22401 NC_009790:c58031-57813 [Escherichia coli O139:H28 str. E24377A]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACCACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References