Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 53912..54513 Replicon plasmid pEF45-2
Accession NZ_CP086606
Organism Escherichia fergusonii strain EF20JDJ4045

Toxin (Protein)


Gene name doc Uniprot ID F0JYA5
Locus tag LNN29_RS23665 Protein ID WP_001216044.1
Coordinates 54133..54513 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag LNN29_RS23660 Protein ID WP_001190712.1
Coordinates 53912..54133 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN29_RS23650 (LNN29_23605) 50895..52283 - 1389 WP_001207923.1 nitrate/nitrite transporter NarU -
LNN29_RS23655 (LNN29_23610) 52546..53526 + 981 WP_000019434.1 IS5-like element ISKpn26 family transposase -
LNN29_RS23660 (LNN29_23615) 53912..54133 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
LNN29_RS23665 (LNN29_23620) 54133..54513 + 381 WP_001216044.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
LNN29_RS23670 (LNN29_23625) 54518..54697 + 180 WP_039022005.1 hypothetical protein -
LNN29_RS23675 (LNN29_23630) 54725..55768 + 1044 WP_125400979.1 DUF968 domain-containing protein -
LNN29_RS23680 (LNN29_23635) 55857..56309 + 453 WP_001326849.1 late promoter-activating protein -
LNN29_RS23685 (LNN29_23640) 56396..57589 + 1194 WP_000219618.1 hypothetical protein -
LNN29_RS23690 (LNN29_23645) 57589..59073 + 1485 WP_125400980.1 terminase -
LNN29_RS23695 (LNN29_23650) 59297..59416 + 120 WP_071527722.1 ash family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaTEM-1B / sul2 - 1..119765 119765
- flank IS/Tn - - 52546..53526 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13544.28 Da        Isoelectric Point: 5.6343

>T224005 WP_001216044.1 NZ_CP086606:54133-54513 [Escherichia fergusonii]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVAATLRRLYGSAE

Download         Length: 381 bp

>T224005 NZ_CP119552:4390166-4390810 [Providencia stuartii]
ATGGCGTATATGAACCTAAACCCAGAAAAAGCACTTATCTGGAGAATAACCCATCGTCAGAATATTCCTTGGCTTTTTGC
CAATGGCTTATATGCAGGAAATAGTGAACATTATTCACCTAATTGGGTGACTATTGGCAATACAGACCTGATTAACCGCC
GCAATCAACGTCATGTACCTATTCAGCCGAATGGGGTACTTAATGATTATGTACCTTTCTATTTCACCCCTTTCTCGCCA
ATGATGTATAATATTTATACAGGCCGTGGTGGGGTGCGAAAAGTTCCCAATGATGATATTATCATCTTGGTTTCCAGCAT
GTATAAAATAGCCGCTCTAAAGATACCTTTTGTTTTCACCGATCGTCATGCGTATCCTGTAACATCTCAGTTCTATAACC
AGCTAACAGATCTCGCGGAGATAGATTGGGCTTTACTACAGCAGCGCAATTTTCACCGTGACCCTGATGACCCTGAAAAA
GTTGAAAGGTATCAAGCAGAAGCGTTAATTTATAAACATGTTCCTATGGAAGCCCTATTAGGTGCTGTTTGTTACACAAA
AGAAACCCAGCTAGAACTTCAAGCAAAAGCCAGCCAACTTGAGATTCATTTAGATATCCATTGCATTCCAAACTGGTACT
TTTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT224005 WP_001190712.1 NZ_CP086606:53912-54133 [Escherichia fergusonii]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT224005 NZ_CP119552:4390822-4391895 [Providencia stuartii]
ATGATAGAATTTACCCAAGGTAATTTGTTAGAAGCTGACGTAGAAGCGCTCGTTAATACCGTCAATACCGTTGGTGTTAT
GGGTAAAGGGATCGCATTAATGTTTAAAGAGCGCTTTCCCGCCAATATGGCTGCATATGCTAAAGCATGTAAAACACATG
AAGTGCAGACTGGAAAAATGTTTGTTACTGAAACCGGGGAGCTCATGGGCCCTCGTTGGATTGTTAATTTCCCAACAAAG
CAGCATTGGCGAGCAAAATCTCAAATTCAGTGGATTATTGATGGCTTATTGGACCTACGTAATTTCATTGAAGAGAATCA
TGTTAAATCTATTGCTATCCCGCCATTAGGGGCTGGTAATGGCGGTCTCAATTGGGATGATGTCAAGCCTCATATTGTGG
AAATATTAGGTGATATTCAAAACGTAAAAATTATTGTTTATGAGCCAATAGCAAAATATCAAAATGTTGCTAAGCCAAAG
GGTGTTGAAAAACTGACGCCGGCCCGCGCCTTAATTGCAGAACTTATCCGTCGTTATTGGGTTATGGGGATGGATTGTAG
CCTATTGGAAATACAAAAGTTAGCTTGGTTTTTAGAGCGTACAATAGAGGCTGAAGGGCTGAGAAATCCATTAAACCTAA
GGTTTGAAGCCAGAAACTATGGTCCCTACGCCGATAAGTTACGACACTTATTAAACGGGCTAGATGGAAGCTACCTCAAG
AGTGAAAAACGGATTAGTGATTGTAGTCCATTAGACGTAATTTGGTTTAATGATGATAAACGCGCTACGATTGAGCTGTA
TTTAAAAACCGAAGCAAAAGACTATTTATCCGCGCTAGAGAAGACAACGAAGCTTATTGATGGATTCGAATCTCCTTTCG
GGATAGAACTTCTCTCCACTGTAGACTGGTTGCTGTACAAAAAGCAATCTTCACCTAATATTGAGTCCTTACTTGAAGGA
ATGCGCCAATGGCCAGCAGGGGATAGATGGGCACAACGTAAACTTAGGTTATTTGACTCAAATAAGTTAGAACTTGCATT
AAATCATTTGAAACAAATACCGTTCTATAGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6Y6HMN5

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References