Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 86741..88364 Replicon plasmid pEF45-1
Accession NZ_CP086605
Organism Escherichia fergusonii strain EF20JDJ4045

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag LNN29_RS22540 Protein ID WP_000088046.1
Coordinates 87039..88364 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag LNN29_RS22535 Protein ID WP_024136327.1
Coordinates 86741..87034 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN29_RS22510 (LNN29_22465) 82613..83854 + 1242 WP_001053338.1 VWA domain-containing protein -
LNN29_RS25260 83945..84076 + 132 WP_000374059.1 hypothetical protein -
LNN29_RS22515 (LNN29_22470) 84417..84698 - 282 WP_000077926.1 hypothetical protein -
LNN29_RS22520 (LNN29_22475) 84748..84939 - 192 WP_000398480.1 hypothetical protein -
LNN29_RS22525 (LNN29_22480) 85031..85372 - 342 WP_001151575.1 hypothetical protein -
LNN29_RS22530 (LNN29_22485) 85745..86137 + 393 WP_000341066.1 hypothetical protein -
LNN29_RS22535 (LNN29_22490) 86741..87034 + 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
LNN29_RS22540 (LNN29_22495) 87039..88364 + 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
LNN29_RS22545 (LNN29_22500) 88425..88631 + 207 WP_000134171.1 hypothetical protein -
LNN29_RS22550 (LNN29_22505) 88733..89143 + 411 WP_000985911.1 hypothetical protein -
LNN29_RS22555 (LNN29_22510) 89156..89401 + 246 Protein_86 HNH endonuclease -
LNN29_RS22560 (LNN29_22515) 89454..90158 - 705 WP_001067858.1 IS6-like element IS26 family transposase -
LNN29_RS22565 (LNN29_22520) 90205..90782 - 578 Protein_88 recombinase family protein -
LNN29_RS22570 (LNN29_22525) 90908..91258 - 351 WP_000454193.1 DUF3330 domain-containing protein -
LNN29_RS22575 (LNN29_22530) 91461..92474 - 1014 WP_000845048.1 class 1 integron integrase IntI1 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aadA2 / cmlA1 / ant(3'')-Ia / sul3 / aac(3)-IVa / aph(4)-Ia / aph(3')-Ia / aph(6)-Id / aph(3'')-Ib / blaTEM-1B / tet(A) / qnrS1 / ARR-3 / blaOXA-10 / dfrA14 / floR - 1..236839 236839


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T224004 WP_000088046.1 NZ_CP086605:87039-88364 [Escherichia fergusonii]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T224004 NZ_CP119552:1536403-1536813 [Providencia stuartii]
GTGGTCCTGTGGAAATCTAATCTTTCCATTTCATGGAAGACACAACTTTTTTCAACCTGTGCACACGGGCTGGTAGGTTT
TATTTTATTGGTAGCACCGTGGGCGCCAGGTAATTCCATGGTCTGGTTACCTTTACTTGCCATTGTGATTGCAAGTTGGG
CTAAAAGCCAAAAAAGTATCAGTAAAATTAAAGGCACCGCAGTGCTCGTGAATGGCAACAAGGTGCAATGGAAAAAAAAC
GAGTGGAATATTATTAAGCAACCGTGGTGTAGTCGTGTTGGTATATTATTGACGCTAAGTGCACTACAAGGTAAGCAACA
AAAAATCCGCTTATGGATAGCAAAGGATGCGTTATCTGAAGAAAGCTGGCGTAATTTAAATCAACTATTACTGCAATATC
CAGATATTTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT224004 WP_024136327.1 NZ_CP086605:86741-87034 [Escherichia fergusonii]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp

>AT224004 NZ_CP119552:1536156-1536422 [Providencia stuartii]
ATGGATATAGAGAACAAAGCAAGAATTCATTGGGCTTGCCGCCGTGGTATGCGTGAATTGGATATTTCCATCATGCCATT
CTTTGAGTATGAGTATGATAGCCTTTCCGATAAGGACAAACAGACTTTCGTCCGTTTACTTGAGTGCGCTGATCCAGACC
TATTTAACTGGTTAATGAATCACGGGCGTCCAGATGATGAAGAGTTGTTTGGTATGGTTAAATTGATTCAGGAAAGAAAT
AAAAATCGTGGTCCTGTGGAAATCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References