Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 4166186..4166769 Replicon chromosome
Accession NZ_CP086604
Organism Escherichia fergusonii strain EF20JDJ4045

Toxin (Protein)


Gene name mazF Uniprot ID V0SV58
Locus tag LNN29_RS20225 Protein ID WP_000254750.1
Coordinates 4166434..4166769 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag LNN29_RS20220 Protein ID WP_000581937.1
Coordinates 4166186..4166434 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN29_RS20210 (4162525) 4162525..4163823 + 1299 WP_104920143.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
LNN29_RS20215 (4163874) 4163874..4166108 + 2235 WP_000226844.1 GTP diphosphokinase -
LNN29_RS20220 (4166186) 4166186..4166434 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
LNN29_RS20225 (4166434) 4166434..4166769 + 336 WP_000254750.1 endoribonuclease MazF Toxin
LNN29_RS20230 (4166840) 4166840..4167631 + 792 WP_001071671.1 nucleoside triphosphate pyrophosphohydrolase -
LNN29_RS20235 (4167859) 4167859..4169496 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
LNN29_RS20240 (4169584) 4169584..4170882 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12067.95 Da        Isoelectric Point: 8.2618

>T224000 WP_000254750.1 NZ_CP086604:4166434-4166769 [Escherichia fergusonii]
MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T224000 NZ_CP119546:1536403-1536813 [Providencia stuartii]
GTGGTCCTGTGGAAATCTAATCTTTCCATTTCATGGAAGACACAACTTTTTTCAACCTGTGCACACGGGCTGGTAGGTTT
TATTTTATTGGTAGCACCGTGGGCGCCAGGTAATTCCATGGTCTGGTTACCTTTACTTGCCATTGTGATTGCAAGTTGGG
CTAAAAGCCAAAAAAGTATCAGTAAAATTAAAGGCACCGCAGTGCTCGTGAATGGCAACAAGGTGCAATGGAAAAAAAAC
GAGTGGAATATTATTAAGCAACCGTGGTGTAGTCGTGTTGGTATATTATTGACGCTAAGTGCACTACAAGGTAAGCAACA
AAAAATCCGCTTATGGATAGCAAAGGATGCGTTATCTGAAGAAAGCTGGCGTAATTTAAATCAACTATTACTGCAATATC
CAGATATTTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT224000 WP_000581937.1 NZ_CP086604:4166186-4166434 [Escherichia fergusonii]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT224000 NZ_CP119546:1536156-1536422 [Providencia stuartii]
ATGGATATAGAGAACAAAGCAAGAATTCATTGGGCTTGCCGCCGTGGTATGCGTGAATTGGATATTTCCATCATGCCATT
CTTTGAGTATGAGTATGATAGCCTTTCCGATAAGGACAAACAGACTTTCGTCCGTTTACTTGAGTGCGCTGATCCAGACC
TATTTAACTGGTTAATGAATCACGGGCGTCCAGATGATGAAGAGTTGTTTGGTATGGTTAAATTGATTCAGGAAAGAAAT
AAAAATCGTGGTCCTGTGGAAATCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SV58

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References