Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3926465..3927015 Replicon chromosome
Accession NZ_CP086604
Organism Escherichia fergusonii strain EF20JDJ4045

Toxin (Protein)


Gene name ccdB Uniprot ID F0JPU1
Locus tag LNN29_RS19140 Protein ID WP_001160963.1
Coordinates 3926701..3927015 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag LNN29_RS19135 Protein ID WP_000125566.1
Coordinates 3926465..3926698 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN29_RS19110 (3921537) 3921537..3921824 + 288 WP_000203741.1 ferredoxin-like protein FixX -
LNN29_RS19115 (3921883) 3921883..3923214 + 1332 WP_104919955.1 MFS transporter -
LNN29_RS19120 (3923322) 3923322..3923852 + 531 WP_000600701.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
LNN29_RS19125 (3923845) 3923845..3925707 + 1863 WP_104919956.1 glutathione-regulated potassium-efflux system protein KefC -
LNN29_RS19130 (3925900) 3925900..3926379 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
LNN29_RS19135 (3926465) 3926465..3926698 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
LNN29_RS19140 (3926701) 3926701..3927015 + 315 WP_001160963.1 CcdB family protein Toxin
LNN29_RS19145 (3927012) 3927012..3927860 - 849 WP_000257189.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
LNN29_RS19150 (3927867) 3927867..3928244 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
LNN29_RS19155 (3928247) 3928247..3929068 - 822 WP_001065388.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
LNN29_RS19160 (3929065) 3929065..3930054 - 990 WP_104918215.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
LNN29_RS19165 (3930054) 3930054..3931340 - 1287 WP_104919957.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11722.70 Da        Isoelectric Point: 8.0666

>T223999 WP_001160963.1 NZ_CP086604:3926701-3927015 [Escherichia fergusonii]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSHIRPPERLNPILLLVDGKEYVLMTHETATVPVNTLG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T223999 NZ_CP119546:c917940-917737 [Providencia stuartii]
ATGACCAAAACTGATTATCTGATGCGTTTAAGAAAATGCACAACCATCGATACACTTGAGCGTGTTATTGAAAAAAATAA
GTATGAGCTTTCTGAAGACGAGCTGGAACTATTTTACTCAGCCGCAGATCACCGTCTTGCAGAACTCACTATGAATAAAC
TTTATGACAAAATTCCAGCCTCTGTTTGGAAATTTGTTAGATAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT223999 WP_000125566.1 NZ_CP086604:3926465-3926698 [Escherichia fergusonii]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT223999 NZ_CP119546:c918387-918019 [Providencia stuartii]
ATGGATGAATATTCACCCAAAAATTATGATATATCAGAGTTAAAATACTTGTGTAACAGTTTGAATAGAGAAGCAATTTC
AAGCTTACAGAAAACAAATACACATTGGATAAATGATTTAAGCTCACCACAAAGTGTACGGCTCAATGAGCTTATTGAAC
ATATAGCCGCCTTTGTATGGCAATATAAGATCAAACATCCAAAAGATAATCTCGTTATATCATTAGTAGAAGAGTATCTT
GACGAGACTTACGATTTATTCGGTAGTCCGGTAATAACGTTAAGTGAAATTATTGACTGGCAGAGTATGAATCAAAATCT
TGTCGCTGTACTTGATGATGATTTAAAATGCCCTGCAAGCAAGACCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB F0JPU1

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References