Detailed information of TA system TA223974

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 16156..16727
• Replicon: plasmid pFac74-1
• Accession: NZ_CP086593
• Organism: Enterococcus faecalis strain Fac74

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A2Z2CGM4
• Locus tag: LNB41_RS14920
• Protein ID: WP_002362432.1
• Coordinates: 16386..16727 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: R3KHK9
• Locus tag: LNB41_RS14915
• Protein ID: WP_002362431.1
• Coordinates: 16156..16386 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNB41_RS14880 (LNB41_14875)	11580..12896	-	1317	WP_229023988.1	Y-family DNA polymerase	-
LNB41_RS14885 (LNB41_14880)	13012..13263	-	252	WP_105223231.1	hypothetical protein	-
LNB41_RS15270	13356..13484	+	129	WP_258077569.1	hypothetical protein	-
LNB41_RS14890 (LNB41_14885)	13600..13950	-	351	WP_225583393.1	SH3 domain-containing protein	-
LNB41_RS14895 (LNB41_14890)	14018..14227	-	210	WP_002358191.1	hypothetical protein	-
LNB41_RS15275	14552..14686	-	135	WP_256213937.1	hypothetical protein	-
LNB41_RS14900 (LNB41_14895)	14819..15034	-	216	WP_105223228.1	hypothetical protein	-
LNB41_RS14905 (LNB41_14900)	15028..15315	-	288	WP_105223227.1	hypothetical protein	-
LNB41_RS14910 (LNB41_14905)	15332..15952	-	621	WP_025189617.1	recombinase family protein	-
LNB41_RS14915 (LNB41_14910)	16156..16386	+	231	WP_002362431.1	hypothetical protein	Antitoxin
LNB41_RS14920 (LNB41_14915)	16386..16727	+	342	WP_002362432.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LNB41_RS14925 (LNB41_14920)	16839..17777	+	939	WP_002362433.1	hypothetical protein	-
LNB41_RS14930 (LNB41_14925)	18042..18644	+	603	WP_229023989.1	Fic family protein	-
LNB41_RS14935 (LNB41_14930)	18898..19659	-	762	WP_229023990.1	hypothetical protein	-
LNB41_RS14940 (LNB41_14935)	19850..20035	+	186	WP_229023997.1	hypothetical protein	-
LNB41_RS14945 (LNB41_14940)	20065..20745	-	681	WP_013646119.1	IS6-like element IS1216 family transposase	-
LNB41_RS14950 (LNB41_14945)	20933..21325	-	393	WP_000084745.1	pyridoxamine 5'-phosphate oxidase family protein	-
LNB41_RS14955 (LNB41_14950)	21577..21708	-	132	Protein_29	IS1380 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	erm(A) / optrA / fexA	asa1	1..69345	69345

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13244.44 Da
• Isoelectric Point: 8.0113

>T223974 WP_002362432.1 NZ_CP086593:16386-16727 [Enterococcus faecalis]
MSDEKKYIPKKGDIVWIDFDPSAEKEIQKRRPGLVVSRYEFNRKTLFAVICPITSTIKNMPTRYTLPDEMETHGQVVISQ
LKSLDFAERKLSQIEHLPLKDMAKIDQIIEYIF

Nucleotide

• Length: 342 bp

>T223974 NZ_CP119528:c2896430-2896329 [Enterococcus faecalis]
TTGTCAATCGAAGCGACATTGGAATTGATGATTAGTTTTGCAACCCTTGTTGCGTTACTGATTTTCGGTATCCTTGAAGC
AACGAAAAACGATAAAAAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8510.42 Da
• Isoelectric Point: 4.3855

>AT223974 WP_002362431.1 NZ_CP086593:16156-16386 [Enterococcus faecalis]
MLTTKSRKQGSSVVLTLPSNNGQKPKADQEYIVMYSDDGTITLVPKLQDPFSGGSEGEYYEKDEWHDIEPGGRELF

Nucleotide

• Length: 231 bp

>AT223974 NZ_CP119528:2896205-2896392 [Enterococcus faecalis]
TGAAAAGAGAGATATGCTACTACATACCTCTCCTTTATACCAACACCAGTTATAAGAACTGGTGGCTTACTGAGTTAAGT
TTTTGTTGTTCTTTAACCGTTCAGCCGTCTAAGCTTATGAACGGTTATTTTTTATCGTTTTTCGTTGCTTCAAGGATACC
GAAAATCAGTAACGCAACAAGGGTTGCA

Structures

Toxin

• AlphaFold DB: A0A2Z2CGM4

Antitoxin

• AlphaFold DB: R3KHK9