Detailed information of TA system TA223967

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 2947901..2948472
• Replicon: chromosome
• Accession: NZ_CP086592
• Organism: Enterococcus faecalis strain Fac74

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: LNB41_RS14360
• Protein ID: WP_002354774.1
• Coordinates: 2947901..2948242 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: R3JGB1
• Locus tag: LNB41_RS14365
• Protein ID: WP_002354773.1
• Coordinates: 2948242..2948472 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNB41_RS14355 (2943916)	2943916..2947530	-	3615	WP_002389470.1	DNA-directed RNA polymerase subunit beta	-
LNB41_RS14360 (2947901)	2947901..2948242	-	342	WP_002354774.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LNB41_RS14365 (2948242)	2948242..2948472	-	231	WP_002354773.1	hypothetical protein	Antitoxin
LNB41_RS14370 (2948886)	2948886..2949101	-	216	WP_002354768.1	zinc ribbon domain-containing protein	-
LNB41_RS14375 (2949240)	2949240..2950232	+	993	WP_002365428.1	biotin--[acetyl-CoA-carboxylase] ligase	-
LNB41_RS14380 (2950299)	2950299..2950931	-	633	WP_002358972.1	RloB family protein	-
LNB41_RS14385 (2950940)	2950940..2952235	-	1296	WP_010820355.1	ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13156.40 Da
• Isoelectric Point: 9.3984

>T223967 WP_002354774.1 NZ_CP086592:c2948242-2947901 [Enterococcus faecalis]
MNVEKKYIPKKGDIVWIDFDPAAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNIPTRYTLPDDIETQGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Nucleotide

• Length: 342 bp

>T223967 NZ_CP119523:c944286-944002 [Pasteurella multocida]
GTGGGTCGGTTAAGGGAGGTAAAAAATTTATCCGCCAAAGCCGCTGTTCTTGCTCGTATTAACAGAGTAATGAATGGTAA
TTTGGGCGATCATAAATCGATTGGAAATGGATTGTATGAGATGAGAATTATAAAAGGTTCGGGTTATAGAGTTTACTATG
GACAATATAGAGAAGTAACTTATTTATTAATTTGTGGTGGCGATAAATCAACACAGAAATCAGACATTGTTAAAGCGAGA
GAGTTGTGGAAAGAAATAAAGCAACAAGAGGAAGTAAAAGTATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.67 Da
• Isoelectric Point: 4.3313

>AT223967 WP_002354773.1 NZ_CP086592:c2948472-2948242 [Enterococcus faecalis]
MLTTKSRRQGSSVVLTLPTDNGKKPKVDQEYIVMYSDDGTITLVPKIQDPFSEGPEGEYYEKDEWHDLAPEGRELF

Nucleotide

• Length: 231 bp

>AT223967 NZ_CP119523:c944005-943712 [Pasteurella multocida]
ATGAGTAAGAAAAAAATAAAATTAAAACTGTTTGATATTGCTGAACATCTAGACAGTGAAGAAATGATTGCGGCATATTT
AAGTGAAGTTTTAGAAACATGTGATACTAATGAATTTATTTCCGCTTTAGCTGATGTTGTAAGAGCAAAAGGAATGACGG
AGTTAGCAGCAAAAACAGGGTTAAGAAGAGAAAGCCTCTATAAAACATTATCATACGGTAGCAAGCCACGTTTTGATACG
ATAATGAAAATTACGCATTCATTAGGTATTAAACTTGTCCCAACGCACATCTAA

Structures

Antitoxin

• AlphaFold DB: A0A0M2A812