22396

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 13101..13744 Replicon plasmid pETEC_73
Accession NC_009788
Organism Escherichia coli E24377A

Toxin (Protein)


Gene name vagD Uniprot ID Q2VNX7
Locus tag ECE24377A_RS00800 Protein ID WP_001044769.1
Coordinates 13328..13744 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag ECE24377A_RS00795 Protein ID WP_001261287.1
Coordinates 13101..13331 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECE24377A_RS00765 8125..8517 - 393 WP_000272015.1 plasmid partitioning/stability family protein -
ECE24377A_RS00770 8521..9495 - 975 WP_001217833.1 hypothetical protein -
ECE24377A_RS30705 9734..10108 - 375 WP_000752652.1 hypothetical protein -
ECE24377A_RS00780 10108..10740 - 633 WP_000312331.1 ParA family protein -
ECE24377A_RS00785 11342..12127 - 786 WP_001164207.1 site-specific integrase -
ECE24377A_RS00790 12129..12542 - 414 WP_000465043.1 hypothetical protein -
ECE24377A_RS00795 13101..13331 + 231 WP_001261287.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
ECE24377A_RS00800 13328..13744 + 417 WP_001044769.1 type II toxin-antitoxin system VapC family toxin Toxin
ECE24377A_RS00805 13906..16044 - 2139 WP_000350636.1 AAA family ATPase -
ECE24377A_RS00810 16360..17898 - 1539 WP_000998104.1 IS66 family transposase -
ECE24377A_RS00815 17948..18295 - 348 WP_000612591.1 IS66 family insertion sequence element accessory protein TnpB -
ECE24377A_RS00820 18292..18672 - 381 WP_001171554.1 IS66 family insertion sequence hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - csbD / csdC / cooA / csbB 1..70609 70609


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15183.63 Da        Isoelectric Point: 7.7805

>T22396 WP_001044769.1 NC_009788:13328-13744 [Escherichia coli O139:H28 str. E24377A]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKVKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T22396 NC_009788:13328-13744 [Escherichia coli O139:H28 str. E24377A]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGGTTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGGGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT22396 WP_001261287.1 NC_009788:13101-13331 [Escherichia coli O139:H28 str. E24377A]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT22396 NC_009788:13101-13331 [Escherichia coli O139:H28 str. E24377A]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A7ZGW5

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References