Detailed information of TA system TA223953

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-DinJ
• Location: 818130..818664
• Replicon: chromosome
• Accession: NZ_CP086592
• Organism: Enterococcus faecalis strain Fac74

Toxin (Protein)

• Gene name: relE
• Uniprot ID: R3JX52
• Locus tag: LNB41_RS04025
• Protein ID: WP_002360769.1
• Coordinates: 818389..818664 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: R3H6P0
• Locus tag: LNB41_RS04020
• Protein ID: WP_002369771.1
• Coordinates: 818130..818396 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNB41_RS03985 (813988)	813988..814773	+	786	WP_048945223.1	replication-relaxation family protein	-
LNB41_RS03990 (814779)	814779..815267	+	489	WP_048945224.1	hypothetical protein	-
LNB41_RS03995 (815859)	815859..816125	+	267	WP_002377947.1	hypothetical protein	-
LNB41_RS04000 (816158)	816158..816658	+	501	WP_002360775.1	DnaJ domain-containing protein	-
LNB41_RS04005 (816671)	816671..816919	+	249	WP_002360773.1	DUF3850 domain-containing protein	-
LNB41_RS04010 (816995)	816995..817411	+	417	WP_010710134.1	single-stranded DNA-binding protein	-
LNB41_RS04015 (817435)	817435..818019	+	585	WP_002377949.1	thermonuclease family protein	-
LNB41_RS04020 (818130)	818130..818396	+	267	WP_002369771.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
LNB41_RS04025 (818389)	818389..818664	+	276	WP_002360769.1	type II toxin-antitoxin system YafQ family toxin	Toxin
LNB41_RS04030 (818855)	818855..819499	-	645	WP_002377952.1	IS6 family transposase	-
LNB41_RS04035 (819723)	819723..820697	+	975	WP_002355428.1	choloylglycine hydrolase	-
LNB41_RS04040 (821366)	821366..821974	-	609	Protein_777	IS6 family transposase	-
LNB41_RS04045 (822006)	822006..822191	-	186	WP_002358660.1	hypothetical protein	-
LNB41_RS04050 (822221)	822221..822550	+	330	WP_002358661.1	LPXTG cell wall anchor domain-containing protein	-
LNB41_RS04055 (822678)	822678..823616	+	939	WP_002370935.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (7-86)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10732.58 Da
• Isoelectric Point: 9.6918

>T223953 WP_002360769.1 NZ_CP086592:818389-818664 [Enterococcus faecalis]
MLEIFYTNQFKKDFKKAKKQGKNLEKLKEVLVLLQEQQTLPPKYKDHALTGNYIGTRECHIEPDWLLIYKIDGDKLILTL
ARIGSHSELFR

Nucleotide

• Length: 276 bp

>T223953 NZ_CP119409:c1656675-1656310 [Rhizobium gei]
ATGATACTTGTCGATGCCTCTGTCTGGATCGACCATCTTCGCGCGCCCGTCGAAGAATTGCAGGCTCTTTTGGAGAGCAG
CAGCGTCCTGATGCACCCTTTTGTGATCGGAGAGATCGGGTTGGGCAGTATCCCGCGTTACGATTTCACGATGCGCGGTC
TTTCGAAATTTCCTTTGGCAACAAAGGCACGGGACGACGATGTTCTCTATCTGGTTCGTGAACATCGACTCTACGGACGA
GGCGTCGGATATATTGATGTCCATTTACTGGCATCGACTCTTTTGACGCCGGAGGCATGCCTTTGGACCCGCGACAAACG
GCTGGCCGAAATCGCTGCCTCCTTCGGCGTCGGCTACAACCGCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9772.09 Da
• Isoelectric Point: 4.4791

>AT223953 WP_002369771.1 NZ_CP086592:818130-818396 [Enterococcus faecalis]
VATTKKKPIHVNVDENLKEEAEQLFDDLGLNMTSAITIFLKQSINEQAIPFMINKGNKETLQALKDIKEGNVHGGFSSVE
DLMEDLNA

Nucleotide

• Length: 267 bp

>AT223953 NZ_CP119409:c1656869-1656675 [Rhizobium gei]
ATGCGAACCACCGTGACAATTGACGACGCGTTGCTTGCCGATGCCAAGGAATTTTCAGGCATCGAGGAGACGGCTGCCGT
CATAAAAGAGGCGTTGACCGCTCTTGTCCAAAGAGAGGCAGCGCGGCGGCTATCGAGACTGGGAGGCAGCGCGCCGGACA
TCGAGGCACCCCCGCGTCGGCGATTTGGCGCATAA

Structures

Toxin

• AlphaFold DB: A0A0M2AEA7

Antitoxin

• AlphaFold DB: A0A0M2AED7