Detailed information of TA system TA022395

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 25355..25914
• Replicon: plasmid pETEC_35
• Accession: NC_009787
• Organism: Escherichia coli E24377A

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A7ZGU0
• Locus tag: ECE24377A_RS00640
• Protein ID: WP_012000729.1
• Coordinates: 25355..25630 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: C1J8K5
• Locus tag: ECE24377A_RS00645
• Protein ID: WP_001178089.1
• Coordinates: 25630..25914 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECE24377A_RS00610	20408..20830	-	423	WP_000774832.1	plasmid partitioning/stability family protein	-
ECE24377A_RS00615	20833..21813	-	981	WP_001218107.1	plasmid segregation protein ParM	-
ECE24377A_RS00625	22556..23557	-	1002	WP_001393213.1	hypothetical protein	-
ECE24377A_RS26930	23799..23963	+	165	Protein_21	IS91 family transposase	-
ECE24377A_RS00635	24197..25201	-	1005	WP_012000728.1	hypothetical protein	-
ECE24377A_RS00640	25355..25630	-	276	WP_012000729.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ECE24377A_RS00645	25630..25914	-	285	WP_001178089.1	ribbon-helix-helix domain-containing protein	Antitoxin
ECE24377A_RS00650	26436..26885	-	450	WP_012000733.1	hypothetical protein	-
ECE24377A_RS26515	27303..28130	+	828	WP_000993597.1	hypothetical protein	-
ECE24377A_RS30690	28210..28350	-	141	WP_001393153.1	hypothetical protein	-
ECE24377A_RS00660	29051..29908	-	858	WP_000131002.1	incFII family plasmid replication initiator RepA	-
ECE24377A_RS26940	29901..29975	-	75	WP_001365705.1	RepA leader peptide Tap	-
ECE24377A_RS00665	30200..30460	-	261	WP_000083819.1	replication regulatory protein RepA	-
ECE24377A_RS26950	30705..30893	-	189	Protein_31	type I toxin-antitoxin system Hok family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..34367	34367
	-	flank	IS/Tn	-	-	23817..23963	146

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10695.40 Da
• Isoelectric Point: 9.9324

>T22395 WP_012000729.1 NC_009787:c25630-25355 [Escherichia coli O139:H28 str. E24377A]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELTGQTVY
VLRLWHTRESR

Nucleotide

• Length: 276 bp

>T22395 NC_009787:c25630-25355 [Escherichia coli O139:H28 str. E24377A]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCAGC
TGCCGCCAGAACGGTGCAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCAGGTGAGTACGAAATCCGTTATGAACTTACTGGCCAGACTGTCTAT
GTATTGCGCTTGTGGCATACGCGGGAAAGCAGATAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10474.89 Da
• Isoelectric Point: 5.6558

>AT22395 WP_001178089.1 NC_009787:c25914-25630 [Escherichia coli O139:H28 str. E24377A]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT22395 NC_009787:c25914-25630 [Escherichia coli O139:H28 str. E24377A]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: A7ZGU0

Antitoxin

• AlphaFold DB: A0A731IHA8