Detailed information of TA system TA223942

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2079524..2080053
• Replicon: chromosome
• Accession: NZ_CP086574
• Organism: Staphylococcus argenteus strain RIVM_M046968

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: LK342_RS09970
• Protein ID: WP_047528533.1
• Coordinates: 2079524..2079886 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: LK342_RS09975
• Protein ID: WP_000948331.1
• Coordinates: 2079883..2080053 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LK342_RS09950 (LK342_09950)	2076506..2077276	-	771	WP_001041104.1	RNA polymerase sigma factor SigB	-
LK342_RS09955 (LK342_09955)	2077251..2077730	-	480	WP_001190826.1	anti-sigma B factor RsbW	-
LK342_RS09960 (LK342_09960)	2077732..2078058	-	327	WP_001052491.1	anti-sigma factor antagonist	-
LK342_RS09965 (LK342_09965)	2078175..2079176	-	1002	WP_000390825.1	PP2C family protein-serine/threonine phosphatase	-
LK342_RS09970 (LK342_09970)	2079524..2079886	-	363	WP_047528533.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LK342_RS09975 (LK342_09975)	2079883..2080053	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
LK342_RS09980 (LK342_09980)	2080138..2081286	-	1149	WP_047528530.1	alanine racemase	-
LK342_RS09985 (LK342_09985)	2081356..2081715	-	360	WP_000581192.1	holo-ACP synthase	-
LK342_RS09990 (LK342_09990)	2081719..2082222	-	504	WP_047528524.1	PH domain-containing protein	-
LK342_RS09995 (LK342_09995)	2082209..2083801	-	1593	WP_047528522.1	PH domain-containing protein	-
LK342_RS10000 (LK342_10000)	2083794..2084273	-	480	WP_047528519.1	PH domain-containing protein	-
LK342_RS10005 (LK342_10005)	2084431..2085024	-	594	Protein_1930	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13488.72 Da
• Isoelectric Point: 10.1909

>T223942 WP_047528533.1 NZ_CP086574:c2079886-2079524 [Staphylococcus argenteus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDNKMNEVDNALMISLGLNTMAHQKN

Nucleotide

• Length: 363 bp

>T223942 NZ_CP119408:324665-325033 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT223942 WP_000948331.1 NZ_CP086574:c2080053-2079883 [Staphylococcus argenteus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT223942 NZ_CP119408:324447-324668 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1