22394

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 40560..41085 Replicon plasmid pETEC_80
Accession NC_009786
Organism Escherichia coli E24377A

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag ECE24377A_RS00295 Protein ID WP_001159871.1
Coordinates 40780..41085 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID D7GK94
Locus tag ECE24377A_RS00290 Protein ID WP_000829078.1
Coordinates 40560..40778 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECE24377A_RS00265 36107..37057 - 951 WP_001395279.1 virulence factor VirK -
ECE24377A_RS26820 37062..38150 - 1089 WP_012131088.1 putative hexosyltransferase CapU -
ECE24377A_RS00275 38153..38995 - 843 WP_162749921.1 polysaccharide deacetylase family protein -
ECE24377A_RS00280 39239..39781 - 543 WP_000194543.1 hypothetical protein -
ECE24377A_RS00285 39781..40038 - 258 WP_000343095.1 hypothetical protein -
ECE24377A_RS00290 40560..40778 + 219 WP_000829078.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
ECE24377A_RS00295 40780..41085 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
ECE24377A_RS00300 41086..41892 + 807 WP_000016959.1 site-specific integrase -
ECE24377A_RS00305 42070..42714 + 645 WP_001144037.1 ParA family protein -
ECE24377A_RS00310 42801..43109 + 309 WP_000030204.1 molecular chaperone GroEL -
ECE24377A_RS30670 43319..44540 + 1222 Protein_59 IS66 family transposase -
ECE24377A_RS00330 44793..45922 - 1130 Protein_60 IS110 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - eltA / eltB / cs3 / etpB 1..79237 79237


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T22394 WP_001159871.1 NC_009786:40780-41085 [Escherichia coli O139:H28 str. E24377A]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T22394 NC_009786:40780-41085 [Escherichia coli O139:H28 str. E24377A]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8440.51 Da        Isoelectric Point: 6.3212

>AT22394 WP_000829078.1 NC_009786:40560-40778 [Escherichia coli O139:H28 str. E24377A]
MKRRITVTVDSDSYQLLKAYDVNISGLVSTIMQNEARRLRAERWKAENREGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT22394 NC_009786:40560-40778 [Escherichia coli O139:H28 str. E24377A]
ATGAAACGGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACCATCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCAGAAAATCGGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A7ZGP5

References