Detailed information of TA system TA223931

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 117192..117979
• Replicon: chromosome
• Accession: NZ_CP086572
• Organism: Staphylococcus argenteus strain RIVM_M036020

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: A0A7U7PZK3
• Locus tag: LK336_RS00730
• Protein ID: WP_000031103.1
• Coordinates: 117824..117979 (+)
• Length: 52 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: -
• Locus tag: LK336_RS00725
• Protein ID: WP_031787929.1
• Coordinates: 117192..117791 (+)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LK336_RS00705 (113108)	113108..114565	+	1458	WP_000649887.1	ABC transporter permease subunit	-
LK336_RS00710 (114558)	114558..115280	+	723	WP_031787931.1	amino acid ABC transporter ATP-binding protein	-
LK336_RS00715 (115431)	115431..116561	+	1131	WP_031787930.1	tRNA epoxyqueuosine(34) reductase QueG	-
LK336_RS00720 (116563)	116563..117033	+	471	WP_000181399.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
LK336_RS00725 (117192)	117192..117791	+	600	WP_031787929.1	glucosamine-6-phosphate isomerase	Antitoxin
LK336_RS00730 (117824)	117824..117979	+	156	WP_000031103.1	SAS053 family protein	Toxin
LK336_RS00735 (118445)	118445..118846	+	402	WP_000901017.1	hypothetical protein	-
LK336_RS00740 (119017)	119017..120402	+	1386	WP_031787928.1	class II fumarate hydratase	-
LK336_RS00745 (120696)	120696..121517	-	822	WP_000669386.1	RluA family pseudouridine synthase	-
LK336_RS00750 (121679)	121679..122791	+	1113	WP_031787927.1	GAF domain-containing sensor histidine kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 6019.42 Da
• Isoelectric Point: 4.1469

>T223931 WP_000031103.1 NZ_CP086572:117824-117979 [Staphylococcus argenteus]
MSKDKDAKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKSTDEKNSH

Nucleotide

• Length: 156 bp

>T223931 NZ_CP119404:c2417647-2417471 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22447.58 Da
• Isoelectric Point: 5.7220

>AT223931 WP_031787929.1 NZ_CP086572:117192-117791 [Staphylococcus argenteus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDNKKSYFEALGV
PASQIYPIAFEKDAIEFISDKIKTKENKGKLTLQVVSIDEHGKLNVSIRQGLMEAREIFLVVTGANKKEVVEKLYQENGK
TSFEPSDLKSHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT223931 NZ_CP119404:c2417425-2417009 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGATA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7U7PZK3