223896

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Xre-MNT/HTH_26(antitoxin)
Location 1442334..1443442 Replicon chromosome
Accession NZ_CP086566
Organism Enterococcus faecalis strain E512-TC2

Toxin (Protein)


Gene name MNTss Uniprot ID A0A193AUR0
Locus tag LM512_RS06960 Protein ID WP_002378351.1
Coordinates 1442334..1443203 (-) Length 290 a.a.

Antitoxin (Protein)


Gene name Xress Uniprot ID -
Locus tag LM512_RS06965 Protein ID WP_000205227.1
Coordinates 1443218..1443442 (-) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LM512_RS06940 (1438605) 1438605..1439924 - 1320 WP_002338419.1 IS1380-like element ISSsu5 family transposase -
LM512_RS06945 (1440152) 1440152..1440553 - 402 WP_002401582.1 phosphoribosyltransferase family protein -
LM512_RS06950 (1440723) 1440723..1441586 - 864 WP_002294505.1 aminoglycoside nucleotidyltransferase ANT(6)-Ia -
LM512_RS06955 (1441619) 1441619..1442353 - 735 WP_000662263.1 class I SAM-dependent methyltransferase -
LM512_RS06960 (1442334) 1442334..1443203 - 870 WP_002378351.1 nucleotidyltransferase domain-containing protein Toxin
LM512_RS06965 (1443218) 1443218..1443442 - 225 WP_000205227.1 helix-turn-helix transcriptional regulator Antitoxin
LM512_RS06970 (1443585) 1443585..1443671 - 87 Protein_1344 site-specific recombinase -
LM512_RS06975 (1443860) 1443860..1445161 + 1302 Protein_1345 ISL3 family transposase -
LM512_RS06980 (1445470) 1445470..1446273 - 804 WP_002294514.1 lincosamide nucleotidyltransferase Lnu(B) -
LM512_RS06985 (1446327) 1446327..1447811 - 1485 WP_002294513.1 ABC-F type ribosomal protection protein Lsa(E) -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside IScluster/Tn erm(B) / ant(6)-Ia / lnu(B) / lsa(E) / aph(3')-III / aac(6')-aph(2'') - 1430943..1461549 30606
- inside Genomic island ant(6)-Ia / lnu(B) / lsa(E) / aph(3')-III / aac(6')-aph(2'') - 1440554..1457035 16481


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Polymerase, nucleotidyl transferase domain

(12-111)

Antitoxin

Cro/C1-type helix-turn-helix domain

(4-57)


Sequences

Toxin        


Download         Length: 290 a.a.        Molecular weight: 32912.50 Da        Isoelectric Point: 4.7903

>T223896 WP_002378351.1 NZ_CP086566:c1443203-1442334 [Enterococcus faecalis]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYNEVNEIVSEINNFLNEESSDERKQI

Download         Length: 870 bp

>T223896 NZ_CP119400:2639667-2639774 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8365.68 Da        Isoelectric Point: 4.5510

>AT223896 WP_000205227.1 NZ_CP086566:c1443442-1443218 [Enterococcus faecalis]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Download         Length: 225 bp

>AT223896 NZ_CP119400:c2639620-2639554 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A193AUR0

Antitoxin

Source ID Structure

References