Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 1432762..1433899 Replicon chromosome
Accession NZ_CP086566
Organism Enterococcus faecalis strain E512-TC2

Toxin (Protein)


Gene name zeta Uniprot ID -
Locus tag LM512_RS06895 Protein ID WP_002401483.1
Coordinates 1432762..1433625 (-) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag LM512_RS06900 Protein ID WP_000301765.1
Coordinates 1433627..1433899 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LM512_RS06865 (1428560) 1428560..1429327 - 768 WP_002357481.1 ribonuclease HII -
LM512_RS06870 (1429329) 1429329..1430180 - 852 WP_002357480.1 ribosome biogenesis GTPase YlqF -
LM512_RS06875 (1430456) 1430456..1430887 - 432 Protein_1325 2-dehydropantoate 2-reductase -
LM512_RS06880 (1430943) 1430943..1431623 - 681 WP_010710189.1 IS6-like element IS1216 family transposase -
LM512_RS06885 (1431697) 1431697..1431984 - 288 Protein_1327 DnaJ domain-containing protein -
LM512_RS06890 (1432004) 1432004..1432321 - 318 WP_002333463.1 hypothetical protein -
LM512_RS06895 (1432762) 1432762..1433625 - 864 WP_002401483.1 zeta toxin family protein Toxin
LM512_RS06900 (1433627) 1433627..1433899 - 273 WP_000301765.1 antitoxin Antitoxin
LM512_RS06905 (1433916) 1433916..1434131 - 216 WP_002295735.1 peptide-binding protein -
LM512_RS06910 (1434230) 1434230..1435126 - 897 WP_002387620.1 ParA family protein -
LM512_RS06915 (1435229) 1435229..1435489 - 261 Protein_1333 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
LM512_RS06920 (1435659) 1435659..1436396 - 738 WP_002292226.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
LM512_RS06925 (1436521) 1436521..1436616 - 96 WP_001809736.1 23S rRNA methyltransferase attenuation leader peptide -
LM512_RS06930 (1436685) 1436685..1436807 - 123 Protein_1336 peptide-binding protein -
LM512_RS06935 (1436927) 1436927..1438453 - 1527 WP_025191980.1 type IA DNA topoisomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside IScluster/Tn erm(B) / ant(6)-Ia / lnu(B) / lsa(E) / aph(3')-III / aac(6')-aph(2'') - 1430943..1461549 30606


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-213)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32486.06 Da        Isoelectric Point: 6.9965

>T223895 WP_002401483.1 NZ_CP086566:c1433625-1432762 [Enterococcus faecalis]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAILEETQGNVIVIDNDTFKQQHPNFDELA
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSRLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMLQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGL

Download         Length: 864 bp

>T223895 NZ_CP119400:c2417573-2417397 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT223895 WP_000301765.1 NZ_CP086566:c1433899-1433627 [Enterococcus faecalis]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp

>AT223895 NZ_CP119400:c2417351-2416935 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGATA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References