Detailed information of TA system TA223886

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 2666414..2666985
• Replicon: chromosome
• Accession: NZ_CP086564
• Organism: Enterococcus faecalis strain E512-TC1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: LM511_RS12650
• Protein ID: WP_002354774.1
• Coordinates: 2666414..2666755 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: R3JGB1
• Locus tag: LM511_RS12655
• Protein ID: WP_002354773.1
• Coordinates: 2666755..2666985 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LM511_RS12645 (2662429)	2662429..2666043	-	3615	WP_041161638.1	DNA-directed RNA polymerase subunit beta	-
LM511_RS12650 (2666414)	2666414..2666755	-	342	WP_002354774.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LM511_RS12655 (2666755)	2666755..2666985	-	231	WP_002354773.1	hypothetical protein	Antitoxin
LM511_RS12660 (2667159)	2667159..2667374	-	216	WP_002354768.1	zinc ribbon domain-containing protein	-
LM511_RS12665 (2667513)	2667513..2668505	+	993	WP_002413308.1	biotin--[acetyl-CoA-carboxylase] ligase	-
LM511_RS12670 (2668769)	2668769..2669407	-	639	WP_002378863.1	lytic polysaccharide monooxygenase	-
LM511_RS12675 (2670093)	2670093..2671709	+	1617	WP_014525060.1	phosphatase PAP2/LCP family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13156.40 Da
• Isoelectric Point: 9.3984

>T223886 WP_002354774.1 NZ_CP086564:c2666755-2666414 [Enterococcus faecalis]
MNVEKKYIPKKGDIVWIDFDPAAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNIPTRYTLPDDIETQGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Nucleotide

• Length: 342 bp

>T223886 NZ_CP119399:490352-490702 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.67 Da
• Isoelectric Point: 4.3313

>AT223886 WP_002354773.1 NZ_CP086564:c2666985-2666755 [Enterococcus faecalis]
MLTTKSRRQGSSVVLTLPTDNGKKPKVDQEYIVMYSDDGTITLVPKIQDPFSEGPEGEYYEKDEWHDLAPEGRELF

Nucleotide

• Length: 231 bp

>AT223886 NZ_CP119399:490065-490346 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Antitoxin

• AlphaFold DB: A0A0M2A812