Detailed information of TA system TA223870

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 1442334..1443442
• Replicon: chromosome
• Accession: NZ_CP086564
• Organism: Enterococcus faecalis strain E512-TC1

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: A0A193AUR0
• Locus tag: LM511_RS06955
• Protein ID: WP_002378351.1
• Coordinates: 1442334..1443203 (-)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: LM511_RS06960
• Protein ID: WP_000205227.1
• Coordinates: 1443218..1443442 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LM511_RS06935 (1438605)	1438605..1439924	-	1320	WP_002338419.1	IS1380-like element ISSsu5 family transposase	-
LM511_RS06940 (1440152)	1440152..1440553	-	402	WP_002401582.1	phosphoribosyltransferase family protein	-
LM511_RS06945 (1440723)	1440723..1441586	-	864	WP_002294505.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
LM511_RS06950 (1441619)	1441619..1442353	-	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
LM511_RS06955 (1442334)	1442334..1443203	-	870	WP_002378351.1	nucleotidyltransferase domain-containing protein	Toxin
LM511_RS06960 (1443218)	1443218..1443442	-	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
LM511_RS06965 (1443585)	1443585..1443671	-	87	Protein_1343	site-specific recombinase	-
LM511_RS06970 (1443860)	1443860..1445161	+	1302	Protein_1344	ISL3 family transposase	-
LM511_RS06975 (1445470)	1445470..1446273	-	804	WP_002294514.1	lincosamide nucleotidyltransferase Lnu(B)	-
LM511_RS06980 (1446327)	1446327..1447811	-	1485	WP_002294513.1	ABC-F type ribosomal protection protein Lsa(E)	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	erm(B) / ant(6)-Ia / lnu(B) / lsa(E) / aph(3')-III / aac(6')-aph(2'')	-	1430943..1461549	30606
	-	inside	Genomic island	ant(6)-Ia / lnu(B) / lsa(E) / aph(3')-III / aac(6')-aph(2'')	-	1440554..1457035	16481

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32912.50 Da
• Isoelectric Point: 4.7903

>T223870 WP_002378351.1 NZ_CP086564:c1443203-1442334 [Enterococcus faecalis]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYNEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T223870 NZ_CP119370:c2742187-2741999 [Pseudomonas sp. CBSPCBW29]
GTGCAAAGCAGGCTATTGATCAAAGAGCTGCAAGAGGCAGGTTGGGTGCTGGATCGGGTGACGGGCAGCCATCATCTTTT
CACCCATCGCTATAACCCGTACACGATACCGGTGCCCCATCCCAAGAAGGACCTGCCGATGGGCACCGTGCGAAGTATCA
GGAAACGTGCCGGGCTGTTTTCGTTTTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT223870 WP_000205227.1 NZ_CP086564:c1443442-1443218 [Enterococcus faecalis]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT223870 NZ_CP119370:c2741977-2741573 [Pseudomonas sp. CBSPCBW29]
ATGCAATACCCAATCTGTATCGAATGGGGCGATGACTTTGTCGCCACCGGTATCCAGATCCCCGACATTCCAGGTGCGGT
GACCGCCGGGGACAGTTTTGAAGAGGCTTACAACGCGGCGGTCGAAGTGGCGCACATCATGCTGCAAGAGATCGCCGCAG
AAGGCCGGGCGATTCCGATGCCAACGTCGGTGGCGGCCCATCACGATAATGAGGATTACGCCGGCATGGGCTGGGGGATG
TTGGAGCTCGACATCTCGCCGTATCTGGGCAAGACCGAGAAGGTCAATGTGACCTTGCCCGGCTACGTGATCCAGCGTAT
CGACCGTTATGTGCGGGAGCACAAAGTGAAAAGCCGCTCGTCATTTCTGGCCGATGCGGCTTTGGAGAAGTTGGTGCGGT
TGTAA

Structures

Toxin

• AlphaFold DB: A0A193AUR0