Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 25794..26365 Replicon plasmid pE509-3
Accession NZ_CP086563
Organism Enterococcus faecalis strain E509

Toxin (Protein)


Gene name mazF Uniprot ID R3HR30
Locus tag LM510_RS14425 Protein ID WP_001278770.1
Coordinates 26024..26365 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A6B1XRC3
Locus tag LM510_RS14420 Protein ID WP_000958277.1
Coordinates 25794..26024 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LM510_RS14390 (LM510_14380) 22319..22570 - 252 WP_010709159.1 hypothetical protein -
LM510_RS14395 (LM510_14385) 22795..23769 - 975 WP_010785309.1 choloylglycine hydrolase -
LM510_RS14400 (LM510_14390) 23868..24329 - 462 WP_002405301.1 hypothetical protein -
LM510_RS14405 (LM510_14395) 24461..24667 - 207 WP_002405302.1 hypothetical protein -
LM510_RS14410 (LM510_14400) 24669..24953 - 285 WP_002405303.1 hypothetical protein -
LM510_RS14415 (LM510_14405) 24970..25590 - 621 WP_002405304.1 recombinase family protein -
LM510_RS14420 (LM510_14410) 25794..26024 + 231 WP_000958277.1 hypothetical protein Antitoxin
LM510_RS14425 (LM510_14415) 26024..26365 + 342 WP_001278770.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LM510_RS14430 (LM510_14420) 26477..27415 + 939 WP_001053446.1 hypothetical protein -
LM510_RS14435 (LM510_14425) 27662..28264 + 603 WP_000892301.1 Fic family protein -
LM510_RS14440 (LM510_14430) 28977..29243 + 267 WP_000246197.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
LM510_RS14445 (LM510_14435) 29236..29511 + 276 WP_000889655.1 type II toxin-antitoxin system YafQ family toxin -
LM510_RS14450 (LM510_14440) 30390..30716 - 327 WP_000606299.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(O) bsh 1..52092 52092


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13220.44 Da        Isoelectric Point: 8.8595

>T223863 WP_001278770.1 NZ_CP086563:26024-26365 [Enterococcus faecalis]
MSDEKKYIPKKGDIVWIDFDPSAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNMPTRYTLPDEMETHGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Download         Length: 342 bp

>T223863 NZ_CP119368:c2321599-2321348 [Pseudomonas sp. CBSPCGW29]
GTGTCGAAAAATGAAAAGCTGCTGGCCAAACTGCTCAATGAACAATCGGCTTTTACCTGGGCCGAACGGGTAACGCTGCT
GCGGCGACTGGGCTACACGCAAATCGAAGGCGCGGGTAGCCGAGTCAGGTTCGACATAGGCGACCCCAGTGCAATGATCA
CCTTGCACAAGCCTCACCCCGGCAACGAACTGAAACACTACATTCGGCGCCAAATCATCGAACAATTGAAATCAGGAGAA
CTGATTCAGTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8536.45 Da        Isoelectric Point: 4.2412

>AT223863 WP_000958277.1 NZ_CP086563:25794-26024 [Enterococcus faecalis]
MLTTKSRKQGSSVVLTLPSNNGQKPKADQEYIVMYSDDGTITLVPKLQDPFSGGSEGEYYEKDEWYDIEPGGRELF

Download         Length: 231 bp

>AT223863 NZ_CP119368:c2321351-2320995 [Pseudomonas sp. CBSPCGW29]
GTGAACAACCAGCTGAAACACAAAGGCTACATCGGCTCCATCGAAGCCAGCCTCGAAGACAACTGCCTCTTCGGAAAGCT
GCTTTTCATCAAGGCCCTGGTCAGCTACGAGGGGAAAACCGTCGCCGAACTGGATACGGCCTTTTGCACCGCCGTGGACG
ACTACATCGAGACCTGCATGGCCCTTGGGCAAACACCGGAAAAGCCCTGCAAGGGCTCGTTCAACGTCCGTGTCGGCCAT
GACCTCCATTTGGCCGCCGCCCTCGCGGCGACCCGGCAAAAAGTCACGCTGAACGACCTCACACGGCAAGCGCTGAACGA
ATTCCTGCAACATCGCGGCGCACTCGTTGCCGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB R3HR30

Antitoxin

Source ID Structure
AlphaFold DB A0A6B1XRC3

References