Detailed information of TA system TA223857

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 1659960..1661068
• Replicon: chromosome
• Accession: NZ_CP086560
• Organism: Enterococcus faecalis strain E509

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: A0A193AUR0
• Locus tag: LM510_RS07980
• Protein ID: WP_002378351.1
• Coordinates: 1659960..1660829 (-)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: LM510_RS07985
• Protein ID: WP_000205227.1
• Coordinates: 1660844..1661068 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LM510_RS07960 (1656231)	1656231..1657550	-	1320	WP_002338419.1	IS1380-like element ISSsu5 family transposase	-
LM510_RS07965 (1657778)	1657778..1658179	-	402	WP_002401582.1	phosphoribosyltransferase family protein	-
LM510_RS07970 (1658349)	1658349..1659212	-	864	WP_002294505.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
LM510_RS07975 (1659245)	1659245..1659979	-	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
LM510_RS07980 (1659960)	1659960..1660829	-	870	WP_002378351.1	nucleotidyltransferase domain-containing protein	Toxin
LM510_RS07985 (1660844)	1660844..1661068	-	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
LM510_RS07990 (1661211)	1661211..1661297	-	87	Protein_1536	site-specific recombinase	-
LM510_RS07995 (1661486)	1661486..1662787	+	1302	Protein_1537	ISL3 family transposase	-
LM510_RS08000 (1663096)	1663096..1663899	-	804	WP_002294514.1	lincosamide nucleotidyltransferase Lnu(B)	-
LM510_RS08005 (1663953)	1663953..1665437	-	1485	WP_002294513.1	ABC-F type ribosomal protection protein Lsa(E)	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	erm(B) / ant(6)-Ia / lnu(B) / lsa(E) / aph(3')-III / aac(6')-aph(2'') / cat	-	1599521..1721689	122168
	-	inside	IScluster/Tn	erm(B) / ant(6)-Ia / lnu(B) / lsa(E) / aph(3')-III / aac(6')-aph(2'') / cat	-	1648569..1682655	34086

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32912.50 Da
• Isoelectric Point: 4.7903

>T223857 WP_002378351.1 NZ_CP086560:c1660829-1659960 [Enterococcus faecalis]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYNEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T223857 NZ_CP119367:678854-679429 [Pseudomonas sp. CBSPGW29]
ATGGACATCGAAAAACTCAAGGGCCGCCTCGACTTCCTGCGCGAAGCGGAAAAACTCAAGGACGTGCTCAGAAGCGCGCA
CACCTCTTCCGGCCGCACCGAAAGCACCGCGGAACACAGCTGGCGCCTGTGCCTGATGGCAATTACCTTCGGGGATGAAC
TGGCCGGCCTCGACCTGCTGAAAATCCTCAAGATGTGCGTAATCCACGACCTGGGAGAGGCCCTCCACGGCGACATCCCG
GCGGTCCAGCAAGCGCAGTTCCCCAACAAGAGCCAGCAAGAACACGACGACCTGCTGTTGCTGACCCGAGCACTGGATGA
GCCGTTGCGGGTCGAGATTCTGGCCCTGTGGGACGACTACGAAAACGCCCGGTCCGCCGAGGCCAAGGCAGTCAAAGCCC
TGGACAAACTGGAGACCCTGCTGCAACACAACCAGGGCCTGAACCCGCCCGACTTCGACTACGCCTTCAATCTGGCCTAT
GGCAAGAAACACACCAGCGCCGAGCCGATTTTCCAGACACTGCGCGAGATCATCGACCAGGATACAGAAGCAAAAATCCA
GACTCCCAACCGGTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT223857 WP_000205227.1 NZ_CP086560:c1661068-1660844 [Enterococcus faecalis]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT223857 NZ_CP119367:678209-678766 [Pseudomonas sp. CBSPGW29]
ATGTCCGACCTGTTGCTCAAGCCTGCCCTGCCCGGCATTGCGCTGAGACGATGGCGTCTGCTGCACCGCGTCAAACAGAC
TCACGCCGCCGAGTTGTTCCACGTCACCCAGTCGACGATTTCCCGCTGGGAAAGTGGCGTGCAGGCCATGGAGCCTGCGG
CGCACCGACAACTTGAACAGTTGCTGGCCGCACGCCTGGAAGCCGCCGCCGATCAGGCCCTCGCCCGGCTGGTCAAGGAA
AGCCCGCGCCCGATGCACCTGGTGTGTGACCTGACCCACCGTCTGCTGGCTTGCTCGGCCTCCCGTGCCGCCGAGTTCAG
CAGCCCGTTGGCGGAACTTCTGGGGCATTCACTGTGGCGCTTCGTGACGCCGGAAATCCTGCGTATGGAGTCCGCGCTGG
ATGACTCGGGCTGGCGCGACAGCCTCGCCCCACCGGCCCTGGAGTTCATCACCGGCACCAACGACTCCACGGTTGTACCG
ATCCGCCAGAGCCTGTGCCGCTGGACCCGCATCCCGCTGAGCGATGGCACCGCCGCGCGGCTGGTCGAGACGCTCTAA

Structures

Toxin

• AlphaFold DB: A0A193AUR0