Detailed information of TA system TA223852

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-DinJ
• Location: 524314..524848
• Replicon: chromosome
• Accession: NZ_CP086560
• Organism: Enterococcus faecalis strain E509

Toxin (Protein)

• Gene name: relE
• Uniprot ID: R3JX52
• Locus tag: LM510_RS02580
• Protein ID: WP_002360769.1
• Coordinates: 524573..524848 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: R3H6P0
• Locus tag: LM510_RS02575
• Protein ID: WP_002369771.1
• Coordinates: 524314..524580 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LM510_RS02540 (520364)	520364..520957	+	594	WP_002414802.1	replication-relaxation family protein	-
LM510_RS02545 (520963)	520963..521451	+	489	WP_010710133.1	hypothetical protein	-
LM510_RS02550 (522043)	522043..522309	+	267	WP_002377947.1	hypothetical protein	-
LM510_RS02555 (522342)	522342..522842	+	501	WP_002360775.1	DnaJ domain-containing protein	-
LM510_RS02560 (522855)	522855..523103	+	249	WP_002360773.1	DUF3850 domain-containing protein	-
LM510_RS02565 (523179)	523179..523595	+	417	WP_010710134.1	single-stranded DNA-binding protein	-
LM510_RS02570 (523619)	523619..524203	+	585	WP_002377949.1	thermonuclease family protein	-
LM510_RS02575 (524314)	524314..524580	+	267	WP_002369771.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
LM510_RS02580 (524573)	524573..524848	+	276	WP_002360769.1	type II toxin-antitoxin system YafQ family toxin	Toxin
LM510_RS02585 (525039)	525039..525683	-	645	WP_002377952.1	IS6 family transposase	-
LM510_RS02590 (525816)	525816..526698	+	883	Protein_478	IS256 family transposase	-
LM510_RS02595 (527003)	527003..528040	-	1038	WP_002355369.1	PTS sugar transporter subunit IIC	-
LM510_RS02600 (528066)	528066..529004	-	939	WP_002370935.1	2-dehydropantoate 2-reductase	-
LM510_RS02605 (529132)	529132..529461	-	330	WP_002358661.1	LPXTG cell wall anchor domain-containing protein	-
LM510_RS02610 (529491)	529491..529676	+	186	WP_002358660.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	bsh / cylR2 / cylR1 / cylL / cylS / cylM / cylB / cylA / cylI / esp	503225..562369	59144

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (7-86)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10732.58 Da
• Isoelectric Point: 9.6918

>T223852 WP_002360769.1 NZ_CP086560:524573-524848 [Enterococcus faecalis]
MLEIFYTNQFKKDFKKAKKQGKNLEKLKEVLVLLQEQQTLPPKYKDHALTGNYIGTRECHIEPDWLLIYKIDGDKLILTL
ARIGSHSELFR

Nucleotide

• Length: 276 bp

>T223852 NZ_CP119344:c2517412-2517146 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCGGCGAAGTTAGATTTAAAGAAAATAAAACATCCTTATTTAAAGAAGAC
ATTTATAGAAATTGTTGAGACTTTAAAAAATAATCCTTATAAAATAACACAATCCTTTGAAAAATTAGAACCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCTTCAGCACAGGGTCGTCTATACCATAGATGATCGAAATAAAGTAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9772.09 Da
• Isoelectric Point: 4.4791

>AT223852 WP_002369771.1 NZ_CP086560:524314-524580 [Enterococcus faecalis]
VATTKKKPIHVNVDENLKEEAEQLFDDLGLNMTSAITIFLKQSINEQAIPFMINKGNKETLQALKDIKEGNVHGGFSSVE
DLMEDLNA

Nucleotide

• Length: 267 bp

>AT223852 NZ_CP119344:c2517669-2517412 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGTAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATGT
ATCTTGTATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Structures

Toxin

• AlphaFold DB: A0A0M2AEA7

Antitoxin

• AlphaFold DB: A0A0M2AED7