Detailed information of TA system TA223838

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 2975430..2976001
• Replicon: chromosome
• Accession: NZ_CP086558
• Organism: Enterococcus faecalis strain E512

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: LM505_RS14640
• Protein ID: WP_002354774.1
• Coordinates: 2975430..2975771 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: R3JGB1
• Locus tag: LM505_RS14645
• Protein ID: WP_002354773.1
• Coordinates: 2975771..2976001 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LM505_RS14635 (2971445)	2971445..2975059	-	3615	WP_002389470.1	DNA-directed RNA polymerase subunit beta	-
LM505_RS14640 (2975430)	2975430..2975771	-	342	WP_002354774.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LM505_RS14645 (2975771)	2975771..2976001	-	231	WP_002354773.1	hypothetical protein	Antitoxin
LM505_RS14650 (2976415)	2976415..2976630	-	216	WP_002354768.1	zinc ribbon domain-containing protein	-
LM505_RS14655 (2976769)	2976769..2977761	+	993	WP_002365428.1	biotin--[acetyl-CoA-carboxylase] ligase	-
LM505_RS14660 (2977828)	2977828..2978460	-	633	WP_002358972.1	RloB family protein	-
LM505_RS14665 (2978469)	2978469..2979569	-	1101	WP_229022098.1	ATP-binding protein	-
LM505_RS14670 (2979569)	2979569..2979763	-	195	WP_229022099.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13156.40 Da
• Isoelectric Point: 9.3984

>T223838 WP_002354774.1 NZ_CP086558:c2975771-2975430 [Enterococcus faecalis]
MNVEKKYIPKKGDIVWIDFDPAAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNIPTRYTLPDDIETQGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Nucleotide

• Length: 342 bp

>T223838 NZ_CP119341:c2079126-2078824 [Staphylococcus aureus]
ATGGAAGCAAAAGTAATAACAAGATATATTGTATTAATTTTAGCATTAGTCAATCAGTTTTTAGCGAATAAAGGTATAAG
TCCGATACCAGTAGATGAAGAAAGTGTTTCATCGATTATCTTAACAGTTATTGCTTTATACACTACGTATAAAGACAATC
CAACATCACAAGAAGGACGTTGGGCGAATCAAAAATTAAAGAAATATAAAGCTGAAAGTAAATATAGAAAAGCAACAGGT
CAAGCACCTGTCAAAGAAGTAATAGCACCTACAAATATGAACGATACAAATGATTTAGGGTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.67 Da
• Isoelectric Point: 4.3313

>AT223838 WP_002354773.1 NZ_CP086558:c2976001-2975771 [Enterococcus faecalis]
MLTTKSRRQGSSVVLTLPTDNGKKPKVDQEYIVMYSDDGTITLVPKIQDPFSEGPEGEYYEKDEWHDLAPEGRELF

Nucleotide

• Length: 231 bp

>AT223838 NZ_CP119341:2079175-2079299 [Staphylococcus aureus]
ATTTGATATTTATATTATGATGTGTTAATTTATATATAGAAAAAGGGCAACATGCGGAAACATGTCACCCTAGTGAGCCC
GTTAAAAAGACGGTGACCTCTTTTATATGATTAATAAATAACCAT

Structures

Antitoxin

• AlphaFold DB: A0A0M2A812