Detailed information of TA system TA022383

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2199242..2199771
• Replicon: chromosome
• Accession: NC_009782
• Organism: Staphylococcus aureus subsp. aureus Mu3

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: SAHV_RS11185
• Protein ID: WP_000621175.1
• Coordinates: 2199242..2199604 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: SAHV_RS11190
• Protein ID: WP_000948331.1
• Coordinates: 2199601..2199771 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAHV_RS11160	2196221..2196991	-	771	WP_001041106.1	RNA polymerase sigma factor SigB	-
SAHV_RS11165	2196966..2197445	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
SAHV_RS11170	2197447..2197773	-	327	WP_001052491.1	anti-sigma factor antagonist	-
SAHV_RS11175	2197892..2198893	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
SAHV_RS11185	2199242..2199604	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
SAHV_RS11190	2199601..2199771	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
SAHV_RS11195	2199856..2201004	-	1149	WP_001281154.1	alanine racemase	-
SAHV_RS11200	2201070..2201429	-	360	WP_000581197.1	holo-ACP synthase	-
SAHV_RS11205	2201433..2201924	-	492	WP_001205912.1	PH domain-containing protein	-
SAHV_RS11210	2201911..2203494	-	1584	WP_001294626.1	PH domain-containing protein	-
SAHV_RS11215	2203487..2203966	-	480	WP_001287087.1	hypothetical protein	-
SAHV_RS11220	2204174..2204734	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T22383 WP_000621175.1 NC_009782:c2199604-2199242 [Staphylococcus aureus subsp. aureus Mu3]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T22383 NC_009782:c2199604-2199242 [Staphylococcus aureus subsp. aureus Mu3]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT22383 WP_000948331.1 NC_009782:c2199771-2199601 [Staphylococcus aureus subsp. aureus Mu3]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT22383 NC_009782:c2199771-2199601 [Staphylococcus aureus subsp. aureus Mu3]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1