Detailed information of TA system TA223824

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 1586372..1587480
• Replicon: chromosome
• Accession: NZ_CP086558
• Organism: Enterococcus faecalis strain E512

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: A0A193AUR0
• Locus tag: LM505_RS07730
• Protein ID: WP_002378351.1
• Coordinates: 1586372..1587241 (-)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: LM505_RS07735
• Protein ID: WP_000205227.1
• Coordinates: 1587256..1587480 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LM505_RS07710 (1581472)	1581472..1582020	-	549	Protein_1492	DNA topoisomerase	-
LM505_RS15445 (1582110)	1582110..1582220	-	111	Protein_1493	hypothetical protein	-
LM505_RS15450 (1582706)	1582706..1583977	-	1272	Protein_1494	IS1380 family transposase	-
LM505_RS07720 (1584764)	1584764..1585624	-	861	Protein_1495	aminoglycoside 6-adenylyltransferase	-
LM505_RS07725 (1585657)	1585657..1586391	-	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
LM505_RS07730 (1586372)	1586372..1587241	-	870	WP_002378351.1	nucleotidyltransferase domain-containing protein	Toxin
LM505_RS07735 (1587256)	1587256..1587480	-	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
LM505_RS07740 (1587623)	1587623..1587709	-	87	Protein_1499	site-specific recombinase	-
LM505_RS07745 (1587898)	1587898..1589198	+	1301	Protein_1500	ISL3 family transposase	-
LM505_RS07750 (1589507)	1589507..1590310	-	804	WP_002294514.1	lincosamide nucleotidyltransferase Lnu(B)	-
LM505_RS07755 (1590364)	1590364..1591848	-	1485	WP_002294513.1	ABC-F type ribosomal protection protein Lsa(E)	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	ant(6)-Ia / lnu(B) / lsa(E) / aph(3')-III / aac(6')-aph(2'') / erm(B)	-	1583600..1605586	21986
	-	inside	Genomic island	lnu(B) / lsa(E) / aph(3')-III / ant(6)-Ia / aac(6')-aph(2'')	-	1585657..1601072	15415

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32912.50 Da
• Isoelectric Point: 4.7903

>T223824 WP_002378351.1 NZ_CP086558:c1587241-1586372 [Enterococcus faecalis]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYNEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T223824 NZ_CP119338:c1981942-1981790 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACTGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATCTCTGATCAAAATGATCAAGAAAAAAATTCTGAAGAAGACAGTCAGTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT223824 WP_000205227.1 NZ_CP086558:c1587480-1587256 [Enterococcus faecalis]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT223824 NZ_CP119338:c1982566-1981967 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTTCTAGATGAATTAAAGAAAAATGTTG
AAAAATATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACAGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Structures

Toxin

• AlphaFold DB: A0A193AUR0