Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relE-dinJ/YafQ-DinJ |
| Location | 814949..815483 | Replicon | chromosome |
| Accession | NZ_CP086558 | ||
| Organism | Enterococcus faecalis strain E512 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | R3JX52 |
| Locus tag | LM505_RS04015 | Protein ID | WP_002360769.1 |
| Coordinates | 815208..815483 (+) | Length | 92 a.a. |
Antitoxin (Protein)
| Gene name | dinJ | Uniprot ID | R3H6P0 |
| Locus tag | LM505_RS04010 | Protein ID | WP_002369771.1 |
| Coordinates | 814949..815215 (+) | Length | 89 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LM505_RS03975 (810807) | 810807..811592 | + | 786 | WP_048945223.1 | replication-relaxation family protein | - |
| LM505_RS03980 (811598) | 811598..812086 | + | 489 | WP_048945224.1 | hypothetical protein | - |
| LM505_RS03985 (812678) | 812678..812944 | + | 267 | WP_002377947.1 | hypothetical protein | - |
| LM505_RS03990 (812977) | 812977..813477 | + | 501 | WP_002360775.1 | DnaJ domain-containing protein | - |
| LM505_RS03995 (813490) | 813490..813738 | + | 249 | WP_002360773.1 | DUF3850 domain-containing protein | - |
| LM505_RS04000 (813814) | 813814..814230 | + | 417 | WP_010710134.1 | single-stranded DNA-binding protein | - |
| LM505_RS04005 (814254) | 814254..814838 | + | 585 | WP_002377949.1 | thermonuclease family protein | - |
| LM505_RS04010 (814949) | 814949..815215 | + | 267 | WP_002369771.1 | type II toxin-antitoxin system RelB/DinJ family antitoxin | Antitoxin |
| LM505_RS04015 (815208) | 815208..815483 | + | 276 | WP_002360769.1 | type II toxin-antitoxin system YafQ family toxin | Toxin |
| LM505_RS04020 (815674) | 815674..816318 | - | 645 | WP_002377952.1 | IS6 family transposase | - |
| LM505_RS04025 (816542) | 816542..817516 | + | 975 | WP_002355428.1 | choloylglycine hydrolase | - |
| LM505_RS04030 (818185) | 818185..818793 | - | 609 | Protein_775 | IS6 family transposase | - |
| LM505_RS04035 (818825) | 818825..819010 | - | 186 | WP_002358660.1 | hypothetical protein | - |
| LM505_RS04040 (819040) | 819040..819369 | + | 330 | WP_002358661.1 | LPXTG cell wall anchor domain-containing protein | - |
| LM505_RS04045 (819497) | 819497..820435 | + | 939 | WP_002370935.1 | 2-dehydropantoate 2-reductase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Integrative and Conjugative Element | - | prgB/asc10 / bsh / cylR2 / cylR1 / cylL / cylS / cylM / cylB / cylA / cylA / cylI / bsh | 769294..859140 | 89846 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 92 a.a. Molecular weight: 10732.58 Da Isoelectric Point: 9.6918
>T223822 WP_002360769.1 NZ_CP086558:815208-815483 [Enterococcus faecalis]
MLEIFYTNQFKKDFKKAKKQGKNLEKLKEVLVLLQEQQTLPPKYKDHALTGNYIGTRECHIEPDWLLIYKIDGDKLILTL
ARIGSHSELFR
MLEIFYTNQFKKDFKKAKKQGKNLEKLKEVLVLLQEQQTLPPKYKDHALTGNYIGTRECHIEPDWLLIYKIDGDKLILTL
ARIGSHSELFR
Download Length: 276 bp
>T223822 NZ_CP119336:119839-120189 [Vibrio alginolyticus]
ATGTGGAATGTAGTAACTAGACCACTTTTTGACGAGTGGTTTCTATCACTAGATGAAAGCGATCAAATTAATGTCCGCGC
ATGCATCCAGGTTCTTCTTGAAAGAGGGCCTCAGCTGAGCAGACCATACGCTGACACTGTGGAAGATTCAAAGCACAGCA
ACATGAAAGAGTTGCGAGTGCAAAGCAATGGGAAACCTATACGGGCTTTCTTTGCTTTTGACCCAGAAAGAACAGGAATT
ATCTTATGTGGCGGTGATAAAACTGGCGATAAGAGATTCTACAAAAAGATGATTCCGATTGCTGATGCAGAATTTGATGA
GCATTTAGAGGAACTTAAAAATGAAAAGTAA
ATGTGGAATGTAGTAACTAGACCACTTTTTGACGAGTGGTTTCTATCACTAGATGAAAGCGATCAAATTAATGTCCGCGC
ATGCATCCAGGTTCTTCTTGAAAGAGGGCCTCAGCTGAGCAGACCATACGCTGACACTGTGGAAGATTCAAAGCACAGCA
ACATGAAAGAGTTGCGAGTGCAAAGCAATGGGAAACCTATACGGGCTTTCTTTGCTTTTGACCCAGAAAGAACAGGAATT
ATCTTATGTGGCGGTGATAAAACTGGCGATAAGAGATTCTACAAAAAGATGATTCCGATTGCTGATGCAGAATTTGATGA
GCATTTAGAGGAACTTAAAAATGAAAAGTAA
Antitoxin
Download Length: 89 a.a. Molecular weight: 9772.09 Da Isoelectric Point: 4.4791
>AT223822 WP_002369771.1 NZ_CP086558:814949-815215 [Enterococcus faecalis]
VATTKKKPIHVNVDENLKEEAEQLFDDLGLNMTSAITIFLKQSINEQAIPFMINKGNKETLQALKDIKEGNVHGGFSSVE
DLMEDLNA
VATTKKKPIHVNVDENLKEEAEQLFDDLGLNMTSAITIFLKQSINEQAIPFMINKGNKETLQALKDIKEGNVHGGFSSVE
DLMEDLNA
Download Length: 267 bp
>AT223822 NZ_CP119336:120179-120484 [Vibrio alginolyticus]
ATGAAAAGTAACCCATTAAGCGTACTTGACGCAAAAATGTCACCAGAAGCACTTGCTGCCGCTAAAGAAAAAGCAGCGGA
TATGCTTCTTGACATTCGACTTGCTGAGCTTCGAGAGATCACCCATCTATCTCAAGTAGAAATCGCTAAAGCTATGGGCA
TTAGCCAGCCATCAGTTGCCAATCTAGAGAAATCTGGCAAAGATTTACGTCTTAGCTCTCTAAAGAAATACGTCGAAGCG
ACCGGTGGCAAGCTTCGCATTGATGTAGAACTGCCAGACGGAAAACATTTTGGCTTCCCGGTGTGA
ATGAAAAGTAACCCATTAAGCGTACTTGACGCAAAAATGTCACCAGAAGCACTTGCTGCCGCTAAAGAAAAAGCAGCGGA
TATGCTTCTTGACATTCGACTTGCTGAGCTTCGAGAGATCACCCATCTATCTCAAGTAGAAATCGCTAAAGCTATGGGCA
TTAGCCAGCCATCAGTTGCCAATCTAGAGAAATCTGGCAAAGATTTACGTCTTAGCTCTCTAAAGAAATACGTCGAAGCG
ACCGGTGGCAAGCTTCGCATTGATGTAGAACTGCCAGACGGAAAACATTTTGGCTTCCCGGTGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0M2AEA7 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0M2AED7 |