Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3465682..3466300 Replicon chromosome
Accession NZ_CP086556
Organism Escherichia coli strain ESY002

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag C5N07_RS16670 Protein ID WP_001291435.1
Coordinates 3466082..3466300 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag C5N07_RS16665 Protein ID WP_000344800.1
Coordinates 3465682..3466056 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5N07_RS16655 (3460771) 3460771..3461964 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
C5N07_RS16660 (3461987) 3461987..3465136 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
C5N07_RS16665 (3465682) 3465682..3466056 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
C5N07_RS16670 (3466082) 3466082..3466300 + 219 WP_001291435.1 HHA domain-containing protein Toxin
C5N07_RS16675 (3466472) 3466472..3467023 + 552 WP_000102564.1 maltose O-acetyltransferase -
C5N07_RS16680 (3467139) 3467139..3467609 + 471 WP_000136192.1 YlaC family protein -
C5N07_RS16685 (3467773) 3467773..3469323 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
C5N07_RS16690 (3469365) 3469365..3469718 - 354 WP_000878140.1 DUF1428 domain-containing protein -
C5N07_RS16700 (3470097) 3470097..3470408 + 312 WP_000409911.1 MGMT family protein -
C5N07_RS16705 (3470439) 3470439..3471011 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T223810 WP_001291435.1 NZ_CP086556:3466082-3466300 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T223810 NZ_CP119332:3737311-3737529 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT223810 WP_000344800.1 NZ_CP086556:3465682-3466056 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT223810 NZ_CP119332:3736910-3737284 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References