Detailed information of TA system TA223773

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1369414..1370039
• Replicon: chromosome
• Accession: NZ_CP086544
• Organism: Escherichia coli strain Ec-042-T0

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LNN55_RS06715
• Protein ID: WP_000911330.1
• Coordinates: 1369641..1370039 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: LNN55_RS06710
• Protein ID: WP_000450524.1
• Coordinates: 1369414..1369641 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNN55_RS06685 (1365217)	1365217..1365687	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
LNN55_RS06690 (1365687)	1365687..1366259	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
LNN55_RS06695 (1366405)	1366405..1367283	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
LNN55_RS06700 (1367300)	1367300..1368334	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
LNN55_RS06705 (1368547)	1368547..1369260	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
LNN55_RS06710 (1369414)	1369414..1369641	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
LNN55_RS06715 (1369641)	1369641..1370039	+	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
LNN55_RS06720 (1370186)	1370186..1371049	+	864	WP_001267507.1	neutral zinc metallopeptidase	-
LNN55_RS06725 (1371064)	1371064..1373079	+	2016	WP_000829323.1	tRNA cytosine(34) acetyltransferase TmcA	-
LNN55_RS06730 (1373153)	1373153..1373851	+	699	WP_000679823.1	esterase	-
LNN55_RS06735 (1373961)	1373961..1374161	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T223773 WP_000911330.1 NZ_CP086544:1369641-1370039 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T223773 NZ_CP119223:1663411-1663800 [Acinetobacter proteolyticus]
ATGGCTAATTCTGCTTTTGAGTTGCAATACAGTCGTTTTGCGCTTGTACTGCAACTCTTGTTGTTTCTCTTTATTGTGGG
CTTAGTTTATTCATTAGTTACATTTTGGTGGTGGTTACTGAGCTTTGCGATGATGACCATCGCATGGTTATCGTTTTTGC
GGCAACCCCAAATTAGGCGTTTTGAATACTTAGATCACCAAGACTGTTCATTCGAATTTTCTGATCCGACCTTGAAAATT
CAGCGTAGACAGATTGTCAAAATATTGGATCACCAGCTCTATATTGCCCTCTATTTTTCTCATAAAAAGCATAAACCCTG
CATTATTTGGCGGGATCAATTGTCACATTCGCAATGGAAAAAGCTAAAAGTACATGCAAAACTAAGCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT223773 WP_000450524.1 NZ_CP086544:1369414-1369641 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT223773 NZ_CP119223:1663161-1663418 [Acinetobacter proteolyticus]
ATGTCTGATGAAATGACACTGGAAGAGCGTAAGGTTGTATATCGTGCCCGTCGTGGTTTAAAAGAAATCGATGTGTATTT
CGACCCGTACGTTAAAAACTACTATTTAACTGCTGATGCTGCTGAAAAAGCCTTATTTGCCGAGCTCGTCGATCAAGAAG
ACCCAGATTTATTGGATTGGTTTATGGAAGTGGGTGAGCCACCGCGTATTGAGTTAAAAGAGTTTATTCTCAAACTCAAA
CATTATGTGCATGGCTAA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33