Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 901153..901807 Replicon chromosome
Accession NZ_CP086544
Organism Escherichia coli strain Ec-042-T0

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag LNN55_RS04425 Protein ID WP_000244777.1
Coordinates 901400..901807 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag LNN55_RS04420 Protein ID WP_000354046.1
Coordinates 901153..901419 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN55_RS04395 (896441) 896441..897184 + 744 Protein_866 SDR family oxidoreductase -
LNN55_RS04400 (897241) 897241..898674 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
LNN55_RS04405 (898719) 898719..899030 + 312 WP_001679467.1 N(4)-acetylcytidine aminohydrolase -
LNN55_RS04410 (899194) 899194..899853 + 660 WP_000250274.1 hemolysin III family protein -
LNN55_RS04415 (899930) 899930..900910 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
LNN55_RS04420 (901153) 901153..901419 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
LNN55_RS04425 (901400) 901400..901807 + 408 WP_000244777.1 protein YgfX Toxin
LNN55_RS04430 (901847) 901847..902368 - 522 WP_001055874.1 flavodoxin FldB -
LNN55_RS04435 (902480) 902480..903376 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
LNN55_RS04440 (903401) 903401..904111 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LNN55_RS04445 (904117) 904117..905850 + 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T223770 WP_000244777.1 NZ_CP086544:901400-901807 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T223770 NZ_CP119199:c1702251-1701865 [Mycolicibacterium vanbaalenii]
GTGACCGAATACCTCGATCGCGATGACGTATTGACAGCAGGATCGGCCGCAGTCGGCCAGGTCGTCCATGTCAGCGACTA
CGGCCTACTTGACGCTGCCGTGGCACGCCCCCGCGCCACTGTCTTCGGGCTCGACGCCTACCCGGACAACTTCACCAAAG
CCGCCGCCTTGCTTCAGTCATTGGCGCGCAATCACGCCCTGGTGGACGGGAACATGCCGATGGCCTGGGCGGCAGCATGG
ATCTTCCTGCACATCAACGGGATCTCACTGGGTGAGTTCGACGTCGACGACGCCGAAGTATTCATGAACAACGTCGCCAT
CAACGGCGATCTCGAAATCGACTACGTCGCCAATAAGCTCAATTCCTACGCCAAACCGGCACAGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT223770 WP_000354046.1 NZ_CP086544:901153-901419 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT223770 NZ_CP119199:c1702445-1702248 [Mycolicibacterium vanbaalenii]
ATGCCGTCCTTGAATGTCTCGTTCACCGAAGAAGAACTGGCGACCGTCCGCGCAGCGGCCGCCAACGATGAAGTCTCACT
GCGGACGTTCGCACACGCAGCCATTCTCTCTGCCGCGAGCGAGCATCGCCGCCGTGTCGCTGAAGCCGCCCGCATCGTCG
CCGAGCGATCGGCAGAACTCAACCGTCGCCTGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References