223765

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 1639..2104 Replicon plasmid pEc-042-T5-MAC_3
Accession NZ_CP086541
Organism Escherichia coli strain Ec-042-T5-MAC

Toxin (Protein)


Gene name TsxA Uniprot ID A0A5Y6UAV0
Locus tag LNN52_RS23255 Protein ID WP_001230708.1
Coordinates 1639..1890 (+) Length 84 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID M7CCF8
Locus tag LNN52_RS23260 Protein ID WP_000801440.1
Coordinates 1877..2104 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN52_RS23245 (LNN52_23240) 480..995 + 516 WP_001025396.1 J domain-containing protein -
LNN52_RS23250 (LNN52_23245) 1100..1642 + 543 WP_001083908.1 hypothetical protein -
LNN52_RS23255 (LNN52_23250) 1639..1890 + 252 WP_001230708.1 hypothetical protein Toxin
LNN52_RS23260 (LNN52_23255) 1877..2104 + 228 WP_000801440.1 hypothetical protein Antitoxin
LNN52_RS23265 (LNN52_23260) 2496..2795 + 300 WP_000650313.1 hypothetical protein -
LNN52_RS23270 (LNN52_23265) 2887..3252 + 366 WP_000854262.1 hypothetical protein -
LNN52_RS23275 (LNN52_23270) 3527..3700 + 174 WP_071821486.1 type II toxin-antitoxin system HicB family antitoxin -
LNN52_RS23280 (LNN52_23275) 4063..4566 + 504 WP_000781818.1 DNA distortion polypeptide 1 -
LNN52_RS23285 (LNN52_23280) 4570..5799 + 1230 WP_058815700.1 DNA relaxase/nickase TaxC -
LNN52_RS23290 (LNN52_23285) 5876..6169 + 294 WP_000134733.1 sigma factor-like helix-turn-helix DNA-binding protein -
LNN52_RS23295 (LNN52_23290) 6166..6381 + 216 WP_000760375.1 EexN family lipoprotein -
LNN52_RS23300 (LNN52_23295) 6558..6734 + 177 WP_000660496.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..34058 34058


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(8-85)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(7-56)


Sequences

Toxin        


Download         Length: 84 a.a.        Molecular weight: 9750.19 Da        Isoelectric Point: 9.8104

>T223765 WP_001230708.1 NZ_CP086541:1639-1890 [Escherichia coli]
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLRKDDETPVKNLIKEFMLN
ETR

Download         Length: 252 bp

>T223765 NZ_CP119182:c4553828-4553565 [Streptomyces caniscabiei]
GTGAGTGAGTACCGCACCGTCTTCCGGGCCGAGGCTCAGGCCGAACTCCGCAAGGTGCCGCGAGACATGGCTCTCCGTAT
TCTGGCCAAGCTGACTGAGCTGGAGAGCGACCCGATGGGGTTCAACACCACGGCCCTCGTCTCCCAGCCCGACCGGCGCC
GCCTACGGGTAGGCGACTACCGCGTGATCTACACCATCGACAACGGCGAGCTGGTGGTGTGGGTCGTGCATGTCGGGCAT
CGCTCCACCGTCTACGACACCTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8659.98 Da        Isoelectric Point: 8.5105

>AT223765 WP_000801440.1 NZ_CP086541:1877-2104 [Escherichia coli]
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK

Download         Length: 228 bp

>AT223765 NZ_CP119182:c4554090-4553821 [Streptomyces caniscabiei]
ATGACACAGTCCATGCCCATAGAGTCGATCCGCGATGTCCGGGCACACCTGGCCGAGGTGGTGGAGCGGGCCGACCGTGA
CGACGTACCTACCGTCATCACCCGGCGCGGCAAGCAGGTCGCCGCCGTGGTGTCCATCGAGGTGCTGCGTAGATATCAGG
AGTGGGAAGAGCGCGAGATCAACCGGATCATCGACGAGCGTATGGCCAACCCGGCACCCGGCATCCCGATCGAGGACATC
ATGAGGGAGACGCTGGCGCGCAGTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5Y6UAV0

Antitoxin

Source ID Structure
AlphaFold DB A0A5Y6U8W9

References