Detailed information of TA system TA223747

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1369415..1370040
• Replicon: chromosome
• Accession: NZ_CP086538
• Organism: Escherichia coli strain Ec-042-T5-MAC

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LNN52_RS06710
• Protein ID: WP_000911330.1
• Coordinates: 1369642..1370040 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: LNN52_RS06705
• Protein ID: WP_000450524.1
• Coordinates: 1369415..1369642 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNN52_RS06680 (1365218)	1365218..1365688	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
LNN52_RS06685 (1365688)	1365688..1366260	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
LNN52_RS06690 (1366406)	1366406..1367284	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
LNN52_RS06695 (1367301)	1367301..1368335	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
LNN52_RS06700 (1368548)	1368548..1369261	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
LNN52_RS06705 (1369415)	1369415..1369642	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
LNN52_RS06710 (1369642)	1369642..1370040	+	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
LNN52_RS06715 (1370187)	1370187..1371050	+	864	WP_001267507.1	neutral zinc metallopeptidase	-
LNN52_RS06720 (1371065)	1371065..1373080	+	2016	WP_000829323.1	tRNA cytosine(34) acetyltransferase TmcA	-
LNN52_RS06725 (1373154)	1373154..1373852	+	699	WP_000679823.1	esterase	-
LNN52_RS06730 (1373962)	1373962..1374162	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T223747 WP_000911330.1 NZ_CP086538:1369642-1370040 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T223747 NZ_CP119174:745968-746453 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT223747 WP_000450524.1 NZ_CP086538:1369415-1369642 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT223747 NZ_CP119174:745678-745971 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33