Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 901154..901808 Replicon chromosome
Accession NZ_CP086538
Organism Escherichia coli strain Ec-042-T5-MAC

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag LNN52_RS04420 Protein ID WP_000244777.1
Coordinates 901401..901808 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag LNN52_RS04415 Protein ID WP_000354046.1
Coordinates 901154..901420 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN52_RS04390 (896442) 896442..897185 + 744 Protein_865 SDR family oxidoreductase -
LNN52_RS04395 (897242) 897242..898675 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
LNN52_RS04400 (898720) 898720..899031 + 312 WP_001679467.1 N(4)-acetylcytidine aminohydrolase -
LNN52_RS04405 (899195) 899195..899854 + 660 WP_000250274.1 hemolysin III family protein -
LNN52_RS04410 (899931) 899931..900911 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
LNN52_RS04415 (901154) 901154..901420 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
LNN52_RS04420 (901401) 901401..901808 + 408 WP_000244777.1 protein YgfX Toxin
LNN52_RS04425 (901848) 901848..902369 - 522 WP_001055874.1 flavodoxin FldB -
LNN52_RS04430 (902481) 902481..903377 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
LNN52_RS04435 (903402) 903402..904112 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LNN52_RS04440 (904118) 904118..905851 + 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T223744 WP_000244777.1 NZ_CP086538:901401-901808 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T223744 NZ_CP119172:1482271-1483116 [Streptococcus macedonicus]
ATGAATAAAGCTAAGCTAACCGCACTCTGTCATAAAATATCTAAGAATACTGGATTAACCTTTAATTCCGTGATGACCTA
TTACTTTCTAGAAGTGATTTTGAAAAAATTAAGTCAGAGTACCTACTCAAATCACTATATCTTCAAAGGAGGATTCCTAC
TGTCAAATGTTATTGGAGTTGAATCTCGTAGCACGGTTGATATTGATTTTTTGTTCCATCAAATAACACTCTCAGAAGAT
ACTGTGAAGCAGCAACTCAAAGAAATTTTAGCAGATTCAGAAGAAGGTATATCTTTTGTGATTCAATCCATCACAGCTAT
TAAAGAAAGTGATGACTATGGTGGCTATCGAGCAACAATTTTGTGCCAACTTGAGAATATTAAACAAGTTATCCATTTGG
ATATTGCGACAGGCGATGTTGTAACTCCTCAGCCGATTACATACGACTATAAAGCTATTTTTGATGAAGAAAATTTTCCA
ATCATTGCTTATACAATTGAAACAATTCTAGCTGAGAAACTTCAAACCATCTATTCACGTAACTTCTTGAACAGCAGAAG
CAAAGATTTTTATGATGTTTATATTCTATCAAAACTAAAGAAAGAAGACATTGACTTCATTCAGTTGAAAAATGCCTGTC
AGAGAACATTTTCATATCGCGAAACAGAGCTCGATTTTGAAAAGATTATTGAACTACTCGAGAGGTTCAAATCTGACCCT
ATTCAGAATAAACAATGGCAAAATTATTCGAAAAAATATAGCTATACAAAGGGGATTTCCCTTGCAGATGTTCTTGATGA
GATGATTCGTCTCATAGCAGTTCTTATTTCTATAAATTCTGATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT223744 WP_000354046.1 NZ_CP086538:901154-901420 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT223744 NZ_CP119172:1481684-1482274 [Streptococcus macedonicus]
ATGTCAAAAAAAGAGATTCTACTTGAATTTATAGAAAACCACAATGGCATTATCACCTATAAAGATTGTAAAGCTCTAAA
TATTCCAACAATATATTTGACAAGACTAGAAAAAGAAGGAATTCTATACCGCGTCGAAAAAGGAATTTTCCTAACCCAGA
ATGGAGACTACGACGAATACTACTTCTTTCAGTACCGATATCCGAAAGCTGTTTTCTCATATATCTCAGCGCTTTATCTG
CAACAATTTACAGATGAAATTCCTCAATATTTTGACGTAACCATTCCTAGAGGTTACCGTTTTAATACTCCTCCAGCTAA
TCTGAACATTCATTCCGTTTCTAAGGAAAATAGCGAACTAGGAATTACTCTTGTAAAAACCCCTATGGGAAACGAGGTAC
GAGCGTATGATTTCGAACGTATTATCTGTGATTTTGTGATTCATCGAGAAAAAATAGATACTGAGCTTTTTGTCAAAACA
CTTCAACTTTACGGAAACTATCCTAAAAAAAATCTTACAAAACTCTATGAATATGCGACAAAAATGAACACCTTGGACAA
GGTCGAACAAACTCTGGAGGTCCTAGTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References