Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 316246..316832 Replicon chromosome
Accession NZ_CP086538
Organism Escherichia coli strain Ec-042-T5-MAC

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag LNN52_RS01450 Protein ID WP_042014162.1
Coordinates 316246..316476 (+) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag LNN52_RS01455 Protein ID WP_000593555.1
Coordinates 316473..316832 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN52_RS01440 (312283) 312283..315018 + 2736 WP_000149132.1 ribosome-associated ATPase/putative transporter RbbA -
LNN52_RS01445 (315018) 315018..316142 + 1125 WP_001314210.1 ABC transporter permease -
LNN52_RS01450 (316246) 316246..316476 + 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
LNN52_RS01455 (316473) 316473..316832 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
LNN52_RS01460 (316952) 316952..317353 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
LNN52_RS01465 (317359) 317359..318165 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
LNN52_RS01470 (318162) 318162..318926 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
LNN52_RS01475 (318926) 318926..319759 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
LNN52_RS01480 (319756) 319756..320700 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T223740 WP_042014162.1 NZ_CP086538:316246-316476 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T223740 NZ_CP119165:c4684549-4684190 [Citrobacter freundii]
ATGACGAAACCACTTTACTGGGTTGGGCAGGCCAGAAAAGACTTGCTTGCAATGCCAGAACACGTTCGGGATACGTTTGG
GTTTGCTTTTTGGCTTGCGCAGCAGGGTAAACAGCATTCACAGACTAAACCATTAAAAGGATTTGGTGGAGCAGGGGTTC
TGGAGGTCGTTGAGGATTACCACGGTAGCGCCTGGCGGGCGGTTTACACCATCCAGTTGAAAAATGCGGTTTATGTTCTC
CATGTTTTTCAGAAGAAATCCGTTTCCGGGAAGGCAACCCCGAAACCTGAAATTGACCTGATTTATCAGCGGCTCAAGGC
TGCACAGCGCCATGCGCAGGAGAGTGGATATGTCATATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT223740 WP_000593555.1 NZ_CP086538:316473-316832 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT223740 NZ_CP119165:c4684200-4683883 [Citrobacter freundii]
ATGTCATATGAAGTGGAAGCCAGTAGCGGTAATGTTTACGTCGATTTAGGTGCGTATGACGCAGAACAGATGCAGTTGAA
AGCGCAACTGGCAATGGTGATTGGTGAGATTTTGCAACGCAAAGGGTTAACGCAGCAGCAGGCCGCGCAGCTACTGGGAA
TGACGCAGCCTAAACTTTCCAACCTGTTACGCGGTCAGTTTCGAGGTATCAGTGAAGCAAAGATGCTCGAATGCCTGGTG
AGACTGGGCCGTGATGTAGAGATTGTTGTCGGGAAAGAGGGCAATATGTCAGGAAAGCTGAAAGTGAAATTTGGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References