Detailed information of TA system TA223739

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/MqsA(antitoxin)
• Location: 11259..11724
• Replicon: plasmid pEc-042-T5-ESBL_3
• Accession: NZ_CP086535
• Organism: Escherichia coli strain Ec-042-T5-ESBL

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: A0A5Y6UAV0
• Locus tag: LNN41_RS23295
• Protein ID: WP_001230708.1
• Coordinates: 11473..11724 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: M7CCF8
• Locus tag: LNN41_RS23290
• Protein ID: WP_000801440.1
• Coordinates: 11259..11486 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNN41_RS23250 (LNN41_23245)	6629..6805	-	177	WP_000660496.1	hypothetical protein	-
LNN41_RS23255 (LNN41_23250)	6982..7197	-	216	WP_000760375.1	EexN family lipoprotein	-
LNN41_RS23260 (LNN41_23255)	7194..7487	-	294	WP_000134733.1	sigma factor-like helix-turn-helix DNA-binding protein	-
LNN41_RS23265 (LNN41_23260)	7564..8793	-	1230	WP_058815700.1	DNA relaxase/nickase TaxC	-
LNN41_RS23270 (LNN41_23265)	8797..9300	-	504	WP_000781818.1	DNA distortion polypeptide 1	-
LNN41_RS23275 (LNN41_23270)	9663..9836	-	174	WP_071821486.1	type II toxin-antitoxin system HicB family antitoxin	-
LNN41_RS23280 (LNN41_23275)	10111..10476	-	366	WP_000854262.1	hypothetical protein	-
LNN41_RS23285 (LNN41_23280)	10568..10867	-	300	WP_000650313.1	hypothetical protein	-
LNN41_RS23290 (LNN41_23285)	11259..11486	-	228	WP_000801440.1	hypothetical protein	Antitoxin
LNN41_RS23295 (LNN41_23290)	11473..11724	-	252	WP_001230708.1	hypothetical protein	Toxin
LNN41_RS23300 (LNN41_23295)	11721..12263	-	543	WP_001083908.1	hypothetical protein	-
LNN41_RS23305 (LNN41_23300)	12368..12883	-	516	WP_001025396.1	J domain-containing protein	-
LNN41_RS23310 (LNN41_23305)	13522..13872	-	351	WP_000127483.1	hypothetical protein	-
LNN41_RS23315 (LNN41_23310)	13869..14204	-	336	WP_001147119.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..34058	34058

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-69)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9750.19 Da
• Isoelectric Point: 9.8104

>T223739 WP_001230708.1 NZ_CP086535:c11724-11473 [Escherichia coli]
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLRKDDETPVKNLIKEFMLN
ETR

Nucleotide

• Length: 252 bp

>T223739 NZ_CP119165:c4581689-4581387 [Citrobacter freundii]
ATGTCTAAATCATTAAATATCAGGAGCTTTCGGGATACATGGCTTGAAGATTTTTTTGAGCGGGCGACATCGCACAGAAA
AATACCAGCGGATATTCATACTGCATTAGCCAGAAAGCTGGATATCATTAATGCGGCTGTTTCGCATCGGGATTTGCGCT
CGCCACCCGGTAACAGATATGAAGAGTTAACCGGAAAATTACAGGAATATTCTTCAATCAGAGTAAACAAGCAGTACCGA
TTAATATTTAAATGGGTTAACGGTAAGGCCGAAGATGTGTACTTAGATCCACACATCTACTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8659.98 Da
• Isoelectric Point: 8.5105

>AT223739 WP_000801440.1 NZ_CP086535:c11486-11259 [Escherichia coli]
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK

Nucleotide

• Length: 228 bp

>AT223739 NZ_CP119165:c4581360-4581019 [Citrobacter freundii]
ATGAAACAGGCAACCAGAAAACCAACGACTGTGGGTGATATCCTGCAATATGAATACCTGGAGCCGCTCGGACTGAAAAT
TAACGATCTGGCTGAAATACTGCATGTTCATCGCAATACGGTGAGCGCACTGGTCAACAATAATCGCAAATTAACAACGG
ATATGGCGTTTCGTTTGGCCAAAGCGTTCGATACCTCCGTTGATTTCTGGCGTAACTTACAGGCGGCGGTTGACCTGTGG
GAAGTTGAAAATGACATGCGGGCGCAAGAAGAACTGAACCGCATTGTTTCGGTTAAAGATTTTATCGCTCAGCGAAATAG
CGAGTCTAAAAAAGTCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A5Y6UAV0

Antitoxin

• AlphaFold DB: A0A5Y6U8W9