Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4177949..4178446 Replicon chromosome
Accession NZ_CP086532
Organism Escherichia coli strain Ec-042-T5-ESBL

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag LNN41_RS20330 Protein ID WP_001173343.1
Coordinates 4177949..4178122 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID J7RC38
Locus tag LNN41_RS20335 Protein ID WP_001425728.1
Coordinates 4178150..4178446 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN41_RS20320 (4174619) 4174619..4176076 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
LNN41_RS20325 (4176313) 4176313..4177830 + 1518 WP_001295074.1 lysine--tRNA ligase -
LNN41_RS20330 (4177949) 4177949..4178122 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
LNN41_RS20335 (4178150) 4178150..4178446 - 297 WP_001425728.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
LNN41_RS20340 (4178673) 4178673..4178945 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
LNN41_RS20345 (4178957) 4178957..4179187 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
LNN41_RS20350 (4179368) 4179368..4180999 + 1632 WP_001216487.1 sensor histidine kinase -
LNN41_RS20355 (4180996) 4180996..4181715 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T223734 WP_001173343.1 NZ_CP086532:c4178122-4177949 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T223734 NZ_CP119165:2075721-2075824 [Citrobacter freundii]
GGCAGGGTAACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11466.99 Da        Isoelectric Point: 4.2544

>AT223734 WP_001425728.1 NZ_CP086532:c4178446-4178150 [Escherichia coli]
MEGKNKFNTYVVSFNYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT223734 NZ_CP119165:c2075829-2075686 [Citrobacter freundii]
CACATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTT
GGCATTAATGCAGGCTAAGTTACCCTGCCATTTAAGAATAGATGACAGCGCCAGGTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB J7RC38

References