Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3446408..3447026 Replicon chromosome
Accession NZ_CP086532
Organism Escherichia coli strain Ec-042-T5-ESBL

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag LNN41_RS16795 Protein ID WP_001291435.1
Coordinates 3446808..3447026 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag LNN41_RS16790 Protein ID WP_000344800.1
Coordinates 3446408..3446782 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN41_RS16780 (3441497) 3441497..3442690 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LNN41_RS16785 (3442713) 3442713..3445862 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
LNN41_RS16790 (3446408) 3446408..3446782 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
LNN41_RS16795 (3446808) 3446808..3447026 + 219 WP_001291435.1 HHA domain-containing protein Toxin
LNN41_RS16800 (3447198) 3447198..3447749 + 552 WP_000102568.1 maltose O-acetyltransferase -
LNN41_RS16805 (3447865) 3447865..3448335 + 471 WP_000136192.1 YlaC family protein -
LNN41_RS16810 (3448499) 3448499..3450049 + 1551 WP_001372022.1 cyclic-guanylate-specific phosphodiesterase PdeB -
LNN41_RS16815 (3450091) 3450091..3450444 - 354 WP_000878140.1 DUF1428 domain-containing protein -
LNN41_RS16825 (3450823) 3450823..3451134 + 312 WP_000409911.1 MGMT family protein -
LNN41_RS16830 (3451165) 3451165..3451737 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T223731 WP_001291435.1 NZ_CP086532:3446808-3447026 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T223731 NZ_CP119165:289618-289893 [Citrobacter freundii]
ATGAAGGAAAAAGTGTTGTCTTTACGCAAAAAGCAAAAGAACACGCTGGATCAGCTTTTTAAAGCACCGACGCCGCAGGG
AATCAAGTGGTCGGAAATTGAGTCACTGATCAAGGCGTTAGGTGGCGAAATCAAAGAAGGACGAGGCTCGCGATGTAAAT
TTCTTTTAAATAAGAGCATTGCGAGTTTTCATAGACCACATCCTTCTCCGGATACAGATAAAGGCGCGGTCGAAAACGTA
CGTGACTGGTTAACAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT223731 WP_000344800.1 NZ_CP086532:3446408-3446782 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT223731 NZ_CP119165:289890-290249 [Citrobacter freundii]
ATGATCAAACCCAAAACGCCAAACTCAATGGAAATCGCCGGTCAACCTGCGGTGATTAATTATGTCCCTGAGCTTAATGC
GTTTCGCGGTAAGTTCCTGGGATTGTCAGGCTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAGGGGG
AGATCTCCTTACGAGAGTATCTTGATGATTGCTCTGCCGCAGGGATAGAACCTTACGCAAACCCGGAAAAGCTGAAGACG
TTTACTCTGCGCTATCCAGAGTCTTTCGGAGAACGTTTAAGTTTTGCGGCAGCGGAAGAACAGGTATCAGTCAATACATG
GATCATCGAAACGCTCAGCAAAAGTCTCAAACAGGCATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References