Detailed information of TA system TA223713

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/MqsA(antitoxin)
• Location: 17127..17592
• Replicon: plasmid pEc-042-T20-MAC_3
• Accession: NZ_CP086529
• Organism: Escherichia coli strain Ec-042-T20-MAC

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: A0A5Y6UAV0
• Locus tag: LNN58_RS23350
• Protein ID: WP_001230708.1
• Coordinates: 17341..17592 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: M7CCF8
• Locus tag: LNN58_RS23345
• Protein ID: WP_000801440.1
• Coordinates: 17127..17354 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNN58_RS23305 (LNN58_23300)	12497..12673	-	177	WP_000660496.1	hypothetical protein	-
LNN58_RS23310 (LNN58_23305)	12850..13065	-	216	WP_000760375.1	EexN family lipoprotein	-
LNN58_RS23315 (LNN58_23310)	13062..13355	-	294	WP_000134733.1	sigma factor-like helix-turn-helix DNA-binding protein	-
LNN58_RS23320 (LNN58_23315)	13432..14661	-	1230	WP_058815700.1	DNA relaxase/nickase TaxC	-
LNN58_RS23325 (LNN58_23320)	14665..15168	-	504	WP_000781818.1	DNA distortion polypeptide 1	-
LNN58_RS23330 (LNN58_23325)	15531..15704	-	174	WP_071821486.1	type II toxin-antitoxin system HicB family antitoxin	-
LNN58_RS23335 (LNN58_23330)	15979..16344	-	366	WP_000854262.1	hypothetical protein	-
LNN58_RS23340 (LNN58_23335)	16436..16735	-	300	WP_000650313.1	hypothetical protein	-
LNN58_RS23345 (LNN58_23340)	17127..17354	-	228	WP_000801440.1	hypothetical protein	Antitoxin
LNN58_RS23350 (LNN58_23345)	17341..17592	-	252	WP_001230708.1	hypothetical protein	Toxin
LNN58_RS23355 (LNN58_23350)	17589..18131	-	543	WP_001083908.1	hypothetical protein	-
LNN58_RS23360 (LNN58_23355)	18236..18751	-	516	WP_001025396.1	J domain-containing protein	-
LNN58_RS23365 (LNN58_23360)	19390..19740	-	351	WP_000127483.1	hypothetical protein	-
LNN58_RS23370 (LNN58_23365)	19737..20072	-	336	WP_001147119.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..34058	34058

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-73)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9750.19 Da
• Isoelectric Point: 9.8104

>T223713 WP_001230708.1 NZ_CP086529:c17592-17341 [Escherichia coli]
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLRKDDETPVKNLIKEFMLN
ETR

Nucleotide

• Length: 252 bp

>T223713 NZ_CP119149:c2442144-2441884 [Streptomyces sp. AM 3-1-1]
GTGAAGTTCGTCTTCGACGAGTCCGCCTGGGACGACTATGTCTGGTGGCAGACCCAGGACCGCAAGGTGCTCAAGCGGAT
CAACATGCTGCTCCAGGAGATCGCCAGGGACGGCAACGAGGGGAGGGGGAAACCCGAACCGCTCAAGCACGGCTTCCACG
GGTACTGGTCCCGGCGGATCACGGACGAGCACAGGCTCGTCTACAAGATCGTGGAGGACCAGATCCGCGTCGCCGCCTGC
CGGTACCACTACGGGCGCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8659.98 Da
• Isoelectric Point: 8.5105

>AT223713 WP_000801440.1 NZ_CP086529:c17354-17127 [Escherichia coli]
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK

Nucleotide

• Length: 228 bp

>AT223713 NZ_CP119149:c2442392-2442141 [Streptomyces sp. AM 3-1-1]
GTGAAGACGATGTCCTACACCGAATCCCGCAAGCACTACGCCGAGACCCTGGACGCGGTGGTAGACGACCGCGAGGAAGT
GGTGATCACGCGTGCCGGGCACGAGCCCGTCGTGATCGTCGCCCTGGACGACTACCAGGCCCTCAAGGAGACCGCTCACC
TGCTCAAGAGCCCCGAGAACGCGCGGCGTCTGCTCGCGTCGATCGAGCGTCTGGAGAACGGCGGTGGCACACCCAGGGAG
CTCGCCGAGTGA

Structures

Toxin

• AlphaFold DB: A0A5Y6UAV0

Antitoxin

• AlphaFold DB: A0A5Y6U8W9