Detailed information of TA system TA223704

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2356457..2358045
• Replicon: chromosome
• Accession: NZ_CP086526
• Organism: Escherichia coli strain Ec-042-T20-MAC

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: F4NFZ0
• Locus tag: LNN58_RS11450
• Protein ID: WP_001125463.1
• Coordinates: 2356723..2358045 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: LNN58_RS11445
• Protein ID: WP_001296726.1
• Coordinates: 2356457..2356723 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNN58_RS11445 (2356457)	2356457..2356723	+	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
LNN58_RS11450 (2356723)	2356723..2358045	+	1323	WP_001125463.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
LNN58_RS11455 (2358357)	2358357..2358565	+	209	Protein_2246	AraC family transcriptional regulator	-
LNN58_RS11460 (2358997)	2358997..2359560	+	564	WP_001373493.1	type 1 fimbrial major subunit FimA	-
LNN58_RS11465 (2359921)	2359921..2360184	+	264	Protein_2248	fimbria/pilus periplasmic chaperone	-
LNN58_RS11470 (2360186)	2360186..2361499	+	1314	Protein_2249	fimbrial biogenesis outer membrane usher protein	-
LNN58_RS11475 (2361513)	2361513..2362043	+	531	WP_000876784.1	fimbrial protein	-
LNN58_RS11480 (2362056)	2362056..2362559	+	504	WP_000825458.1	fimbrial protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimA	2356457..2372056	15599

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (7-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49422.05 Da
• Isoelectric Point: 9.1203

>T223704 WP_001125463.1 NZ_CP086526:2356723-2358045 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVERFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALRDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK

Nucleotide

• Length: 1323 bp

>T223704 NZ_CP119138:c4635640-4635290 [Alicyclobacillus fastidiosus]
TTGAACGTCAAACGAGGGGACGTTTTCTTTGCGGACTTGTCACCCGTAGTCGGTTCTGAACAAGGCGGCTTTCGCCCCGT
TCTCATCATTCAGAACGACATCGGGAATCGCTTTAGTCCCACCGTCATCGTGGCGGCTATCACGGCCCAAATTCAAAAGG
CGAAACTGCCAACTCACGTCGAGATCCCGGCTCAGCCGTATGGCCTCGAACGGGACTCGGTCGTACTGTTGGAACAGTTG
CGCACGTTGGATAAACAACGTTTGACGGACAAAATCACGCACTTGGACGACACCATGATGAAGATGGTCAATGACGGACT
CCTCATCAGCCTCGGACTTGTGGACTTTTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT223704 WP_001296726.1 NZ_CP086526:2356457-2356723 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT223704 NZ_CP119138:c4635899-4635645 [Alicyclobacillus fastidiosus]
ATGGTAGTCTACGTTCCGGAGCAGTTGCTAGAGCAAGTGGATGGCTTCGCTCGAACGGATAGCGCAAATCGCAGTGAAAT
TGTACGAGAAGCCTTGCGTATGTATGTAACAGCGCACCGTAAGAACGACATTCGCGAACAGATGCAGCAGGGGTATCTGG
AAATGGCTCGATTAAATTTGCGAATTGCTTCCGAATCATTTCCCTTGGAACAAGAAGCAGGAGGCACGGTGGAACGCTTA
GTAAGCGGGGTGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9IYX0