Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 337549..338349 Replicon chromosome
Accession NZ_CP086526
Organism Escherichia coli strain Ec-042-T20-MAC

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag LNN58_RS01585 Protein ID WP_000342449.1
Coordinates 337822..338349 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag LNN58_RS01580 Protein ID WP_001277108.1
Coordinates 337549..337815 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN58_RS01560 (333206) 333206..333874 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
LNN58_RS01565 (333867) 333867..334925 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
LNN58_RS01570 (335170) 335170..336024 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
LNN58_RS01575 (336296) 336296..337399 + 1104 WP_001350438.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
LNN58_RS01580 (337549) 337549..337815 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
LNN58_RS01585 (337822) 337822..338349 + 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
LNN58_RS01590 (338346) 338346..338729 - 384 WP_000778781.1 aspartate 1-decarboxylase autocleavage activator PanM -
LNN58_RS01595 (339153) 339153..340262 + 1110 WP_089455105.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
LNN58_RS01600 (340310) 340310..341236 + 927 WP_000003004.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
LNN58_RS01605 (341233) 341233..342510 + 1278 WP_000803799.1 branched chain amino acid ABC transporter permease LivM -
LNN58_RS01610 (342507) 342507..343274 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T223689 WP_000342449.1 NZ_CP086526:337822-338349 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T223689 NZ_CP119130:c52772-52491 [Sphingobium sp. WTD-1]
ATGCGGACGATTGATCGTTCGACCCGGTTCAAGCGCGACTACAAGCGGGAATCAAAGGGCCGACATCGAGCGACCCTCGA
TGCCGATCTTCTGCCCGTCCTGGCCGCGCTCGCGACCGACACGCCGCTTGATCCCAAATACCGCGACCATGATCTGAGCG
GCGATTGGGCGGGCTACCGCGATTGCCACGTCAAGCCCGATCTGGTGCTGATCTACGCCAAGCCCGACGACGAAACGCTG
CGCCTCGCGCGCCTTGGCTCGCATAGCGAGGTATTCGGATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT223689 WP_001277108.1 NZ_CP086526:337549-337815 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT223689 NZ_CP119130:c53025-52759 [Sphingobium sp. WTD-1]
ATGGCTGTTTCCGACACCTATGTCCGCGCCAGGATCGACAATACGACGAAGGAACGCGCCACCGCCGCGCTGGGCGCGAT
GGGCCTTTCGATCTCCGACGCTATCCGGCTGCTGATGCTGCGTATCGCCGACGAACGGCGCTTGCCTTTCGAGGTCAAGG
TTCCGAACGCCGCGACACGCGAGGCAATGGCAGAGCTGGCGGCGGGGAAGGGGACCAAGTTTGCCAGCGTCGATGCGCTG
ATGGCGGACCTCCATGCGGACGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References