223687

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 9461..9926 Replicon plasmid pEc-042-T20-ESBL_3
Accession NZ_CP086523
Organism Escherichia coli strain Ec-042-T20-ESBL

Toxin (Protein)


Gene name TsxA Uniprot ID A0A5Y6UAV0
Locus tag LNN45_RS23285 Protein ID WP_001230708.1
Coordinates 9675..9926 (-) Length 84 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID M7CCF8
Locus tag LNN45_RS23280 Protein ID WP_000801440.1
Coordinates 9461..9688 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN45_RS23240 (LNN45_23235) 4831..5007 - 177 WP_000660496.1 hypothetical protein -
LNN45_RS23245 (LNN45_23240) 5184..5399 - 216 WP_000760375.1 EexN family lipoprotein -
LNN45_RS23250 (LNN45_23245) 5396..5689 - 294 WP_000134733.1 sigma factor-like helix-turn-helix DNA-binding protein -
LNN45_RS23255 (LNN45_23250) 5766..6995 - 1230 WP_058815700.1 DNA relaxase/nickase TaxC -
LNN45_RS23260 (LNN45_23255) 6999..7502 - 504 WP_000781818.1 DNA distortion polypeptide 1 -
LNN45_RS23265 (LNN45_23260) 7865..8038 - 174 WP_071821486.1 type II toxin-antitoxin system HicB family antitoxin -
LNN45_RS23270 (LNN45_23265) 8313..8678 - 366 WP_000854262.1 hypothetical protein -
LNN45_RS23275 (LNN45_23270) 8770..9069 - 300 WP_000650313.1 hypothetical protein -
LNN45_RS23280 (LNN45_23275) 9461..9688 - 228 WP_000801440.1 hypothetical protein Antitoxin
LNN45_RS23285 (LNN45_23280) 9675..9926 - 252 WP_001230708.1 hypothetical protein Toxin
LNN45_RS23290 (LNN45_23285) 9923..10465 - 543 WP_001083908.1 hypothetical protein -
LNN45_RS23295 (LNN45_23290) 10570..11085 - 516 WP_001025396.1 J domain-containing protein -
LNN45_RS23300 (LNN45_23295) 11724..12074 - 351 WP_000127483.1 hypothetical protein -
LNN45_RS23305 (LNN45_23300) 12071..12406 - 336 WP_001147119.1 type II toxin-antitoxin system RelE/ParE family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..34058 34058


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-74)

Antitoxin

Toxin YoeB

(5-84)


Sequences

Toxin        


Download         Length: 84 a.a.        Molecular weight: 9750.19 Da        Isoelectric Point: 9.8104

>T223687 WP_001230708.1 NZ_CP086523:c9926-9675 [Escherichia coli]
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLRKDDETPVKNLIKEFMLN
ETR

Download         Length: 252 bp

>T223687 NZ_CP119128:400605-400856 [Sphingobium sp. WTD-1]
ATGGACGCTATCACTTACACACACGCCCGCGCCAACCTGGCTGGAACCATGGATCGTGTTTGCAACGATCATGAGCCGGT
GATCATCACACGTAACGGCGACCAATCGGTTGTGATTCTCTCGCTGGAAGATTTCCAGGCGCTGGAAGAAACTGCGTATC
TGCTGCGCAGCCCCGCCAACGCCAAGCGGCTTTTCAAGTCGATCGAGCAACTTGAAGCGGGCCGAGGACAGGCGCGGGAG
TTGGCCGAATGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8659.98 Da        Isoelectric Point: 8.5105

>AT223687 WP_000801440.1 NZ_CP086523:c9688-9461 [Escherichia coli]
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK

Download         Length: 228 bp

>AT223687 NZ_CP119128:400853-401107 [Sphingobium sp. WTD-1]
ATGAGATTAATTTTTTCCGATGATGCATGGGAAGATTATCTGTTCTGGCAAAAACAAGACCGCCGCATGGTTGATCGAAT
TAACAAGCTAATTAAAGAAACCACCCGAGAGCCTTTCTCTGGAGTGGGCAAGCCAGAGCCATTAAAACATGCTTTGGCCG
GTTATTGGTCTCGTCGAATTACGGATGAGCACCGAATGGTTTACAAAGTGGCGGATGATGCCCTTTGGATCGTTCAACTC
AAGTACCACTACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5Y6UAV0

Antitoxin

Source ID Structure
AlphaFold DB A0A5Y6U8W9

References